Detailed information of TA system TA120431

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralR-sib/-
• Location: 3087661..3088032
• Replicon: chromosome
• Accession: NZ_CP037941
• Organism: Escherichia coli strain CFSAN027350

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: A0A839BBC2
• Locus tag: EKI55_RS15660
• Protein ID: WP_021500490.1
• Coordinates: 3087838..3088032 (-)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	sib
Locus tag	-
Coordinates	3087661..3087839 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EKI55_RS15635	3083616..3084989	+	1374	WP_000123745.1	ATP-dependent RNA helicase DbpA	-
EKI55_RS15640	3085118..3086053	-	936	WP_001157377.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
EKI55_RS15645	3086106..3087340	-	1235	Protein_2985	site-specific integrase	-
EKI55_RS15650	3087342..3087557	-	216	WP_000079604.1	excisionase XisR	-
EKI55_RS15655	3087657..3087845	-	189	WP_001298826.1	DUF1187 family protein	-
-	3087661..3087839	+	179	NuclAT_0	-	Antitoxin
-	3087661..3087839	+	179	NuclAT_0	-	Antitoxin
-	3087661..3087839	+	179	NuclAT_0	-	Antitoxin
-	3087661..3087839	+	179	NuclAT_0	-	Antitoxin
EKI55_RS15660	3087838..3088032	-	195	WP_021500490.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
EKI55_RS15665	3088096..3089148	-	1053	WP_001004423.1	recombinase RecT	-
EKI55_RS15670	3089160..3092285	-	3126	WP_000102216.1	exodeoxyribonuclease VIII	-
EKI55_RS15675	3092387..3092662	-	276	WP_001519830.1	phage protein	-
EKI55_RS15680	3092737..3092913	-	177	WP_000245528.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	nleC	3086106..3141516	55410

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7046.98 Da
• Isoelectric Point: 9.2886

>T120431 WP_021500490.1 NZ_CP037941:c3088032-3087838 [Escherichia coli]
MRYEKVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Nucleotide

• Length: 195 bp

>T120431 NZ_CP037941:c3088032-3087838 [Escherichia coli]
ATGCGGTATGAAAAAGTCAAACCATGCCCTTTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
CGCGAAGTGTAACGGATGCGAATCCCGAACCGGCTATGGTGGAAGTGAAAAAGAAGCACTCGAACGATGGAATAAACGAA
CCACTGGAAATAATAATGGAGGTGTTCATGTATAA

Antitoxin

Nucleotide

• Length: 179 bp

>AT120431 NZ_CP037941:3087661-3087839 [Escherichia coli]
GAGGACTGAAGTTGCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTGGTTAATTTTGTTTTTGAGTACCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A839BBC2

Antitoxin