Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 1648020..1648555 Replicon chromosome
Accession NZ_CP037941
Organism Escherichia coli strain CFSAN027350

Toxin (Protein)


Gene name yafQ Uniprot ID A0A0F3UZU8
Locus tag EKI55_RS07975 Protein ID WP_032183258.1
Coordinates 1648020..1648307 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag EKI55_RS07980 Protein ID WP_000729704.1
Coordinates 1648295..1648555 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EKI55_RS07960 1645602..1646180 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
EKI55_RS07965 1646386..1647153 + 768 WP_032345349.1 class II glutamine amidotransferase -
EKI55_RS07970 1647124..1647864 - 741 WP_001225679.1 murein L,D-transpeptidase -
EKI55_RS07975 1648020..1648307 - 288 WP_032183258.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
EKI55_RS07980 1648295..1648555 - 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
EKI55_RS07985 1648741..1649514 + 774 WP_000543897.1 C40 family peptidase -
EKI55_RS07990 1649571..1649927 - 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
EKI55_RS07995 1649920..1650198 - 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
EKI55_RS08000 1650303..1652396 - 2094 WP_001140806.1 flagellar biosynthesis protein FlhA -
EKI55_RS08005 1652380..1653519 - 1140 WP_000785656.1 flagellar type III secretion system protein FlhB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(7-94)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11166.96 Da        Isoelectric Point: 10.2267

>T120425 WP_032183258.1 NZ_CP037941:c1648307-1648020 [Escherichia coli]
IRNLNQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDK
LLRFERTGTHAALFG

Download         Length: 288 bp

>T120425 NZ_CP037941:c1648307-1648020 [Escherichia coli]
ATTAGGAATTTAAATCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAA
GGATATGAATAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACC
CGCTGCAAGGTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAA
CTTTTACGATTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT120425 WP_000729704.1 NZ_CP037941:c1648555-1648295 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT120425 NZ_CP037941:c1648555-1648295 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0F3UZU8

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References