Detailed information of TA system TA120419

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 385008..385610
• Replicon: chromosome
• Accession: NZ_CP037941
• Organism: Escherichia coli strain CFSAN027350

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: EKI55_RS01890
• Protein ID: WP_000897305.1
• Coordinates: 385299..385610 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: EKI55_RS01885
• Protein ID: WP_000356397.1
• Coordinates: 385008..385298 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EKI55_RS01860	380933..381835	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
EKI55_RS01865	381832..382467	+	636	WP_000829008.1	formate dehydrogenase cytochrome b556 subunit	-
EKI55_RS01870	382464..383393	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
EKI55_RS01875	383724..383966	-	243	WP_001086388.1	hypothetical protein	-
EKI55_RS01880	384185..384403	-	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
EKI55_RS01885	385008..385298	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
EKI55_RS01890	385299..385610	-	312	WP_000897305.1	hypothetical protein	Toxin
EKI55_RS01895	385839..386747	+	909	WP_001162704.1	alpha/beta hydrolase	-
EKI55_RS01900	386811..387752	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
EKI55_RS01905	387797..388234	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
EKI55_RS01910	388231..389103	-	873	WP_000920762.1	virulence factor BrkB family protein	-
EKI55_RS01915	389097..389696	-	600	WP_001315111.1	glucose-1-phosphatase	-
EKI55_RS01920	389795..390580	-	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T120419 WP_000897305.1 NZ_CP037941:c385610-385299 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T120419 NZ_CP037941:c385610-385299 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT120419 WP_000356397.1 NZ_CP037941:c385298-385008 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT120419 NZ_CP037941:c385298-385008 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7