Detailed information of TA system TA120418

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 8769..9426
• Replicon: plasmid p36788
• Accession: NZ_CP037935
• Organism: Marinobacter sp. JH2 strain sp.

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: MARI_RS16820
• Protein ID: WP_133007692.1
• Coordinates: 8769..9122 (+)
• Length: 118 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: MARI_RS16825
• Protein ID: WP_133007693.1
• Coordinates: 9124..9426 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MARI_RS16805	3918..7103	+	3186	WP_133007689.1	type I restriction endonuclease subunit R	-
MARI_RS16810	7100..7525	+	426	WP_133007690.1	hypothetical protein	-
MARI_RS16815	8154..8621	+	468	WP_133007691.1	helix-turn-helix domain-containing protein	-
MARI_RS16820	8769..9122	+	354	WP_133007692.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MARI_RS16825	9124..9426	+	303	WP_133007693.1	XRE family transcriptional regulator	Antitoxin
MARI_RS16830	9495..11360	-	1866	WP_165950637.1	relaxase/mobilization nuclease domain-containing protein	-
MARI_RS16835	11344..11691	-	348	WP_133007695.1	plasmid mobilization relaxosome protein MobC	-
MARI_RS16840	11900..12313	-	414	WP_133007696.1	hypothetical protein	-
MARI_RS16845	12318..12545	-	228	WP_133007697.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..36788	36788

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• HigA2-like, helix-turn-helix domain (19-89)

Sequences

Toxin

Protein

• Length: 118 a.a.
• Molecular weight: 13417.28 Da
• Isoelectric Point: 7.1661

>T120418 WP_133007692.1 NZ_CP037935:8769-9122 [Marinobacter sp. JH2]
MWEVKTTDRFDEWYKTLDDTDRKNVLAAILVLQGKGPMLSRPYADSVYGSKHSNMKELRVQSKGNPIRAFFAFDPTRAGI
ILCAGEKTGKEKRFYDEMIPVADDEFSAHLEELQRGK

Nucleotide

• Length: 354 bp

>T120418 NZ_CP037935:8769-9122 [Marinobacter sp. JH2]
ATGTGGGAAGTAAAAACGACTGATAGATTTGATGAGTGGTACAAAACTCTCGACGACACAGATAGAAAAAACGTTCTTGC
AGCAATCTTAGTATTGCAGGGAAAGGGACCAATGCTATCGCGACCATACGCAGATAGCGTGTACGGTTCCAAACACAGCA
ACATGAAAGAGTTGCGCGTTCAAAGCAAAGGCAATCCAATCCGAGCATTCTTTGCGTTTGATCCAACCCGGGCAGGCATC
ATTCTCTGTGCCGGTGAGAAAACCGGAAAAGAGAAACGGTTTTATGATGAGATGATTCCAGTAGCCGATGATGAATTTTC
GGCACACCTGGAGGAACTCCAACGAGGTAAATAG

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 10889.69 Da
• Isoelectric Point: 8.5943

>AT120418 WP_133007693.1 NZ_CP037935:9124-9426 [Marinobacter sp. JH2]
MANSLDKLLANEKPEVVKRAQAKAESTLLEIHLAEVRALLQKTQKDMAEAMGVTQPTIAGMEKFGKDLKLSSVKRYVEGA
GAKVRLDIELPDGTHHAVPL

Nucleotide

• Length: 303 bp

>AT120418 NZ_CP037935:9124-9426 [Marinobacter sp. JH2]
ATGGCTAATTCTCTGGACAAGCTTTTAGCTAACGAAAAACCAGAGGTTGTAAAACGAGCTCAGGCCAAGGCTGAAAGCAC
TCTACTTGAGATTCATCTGGCTGAAGTTCGTGCGTTACTACAAAAAACACAAAAAGACATGGCTGAAGCTATGGGAGTAA
CTCAACCAACAATTGCTGGTATGGAAAAATTTGGAAAAGATTTAAAACTATCTTCTGTGAAGCGCTATGTAGAAGGCGCT
GGCGCCAAAGTACGCCTGGATATTGAACTGCCAGATGGAACGCACCACGCGGTTCCTTTGTGA