Detailed information of TA system TA120397

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 5349905..5350500
• Replicon: chromosome
• Accession: NZ_CP037926
• Organism: Pseudomonas aeruginosa strain AES1R

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: CKAES1R_RS25720
• Protein ID: WP_003113526.1
• Coordinates: 5350222..5350500 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CKAES1R_RS25715
• Protein ID: WP_003113527.1
• Coordinates: 5349905..5350210 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CKAES1R_RS25680	5345045..5345893	+	849	WP_003146058.1	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase	-
CKAES1R_RS25690	5346060..5347001	+	942	WP_003099281.1	ribose-phosphate pyrophosphokinase	-
CKAES1R_RS25695	5347118..5347732	+	615	WP_003099279.1	50S ribosomal protein L25/general stress protein Ctc	-
CKAES1R_RS25700	5347774..5348358	+	585	WP_003099278.1	aminoacyl-tRNA hydrolase	-
CKAES1R_RS25705	5348399..5349499	+	1101	WP_003099270.1	redox-regulated ATPase YchF	-
CKAES1R_RS25715	5349905..5350210	-	306	WP_003113527.1	HigA family addiction module antidote protein	Antitoxin
CKAES1R_RS25720	5350222..5350500	-	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CKAES1R_RS25730	5350829..5353057	+	2229	WP_003113525.1	TonB-dependent receptor	-
CKAES1R_RS25735	5353127..5353774	-	648	WP_003095021.1	carbonate dehydratase	-
CKAES1R_RS25740	5353836..5355074	-	1239	WP_019681675.1	C69 family dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (22-69)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T120397 WP_003113526.1 NZ_CP037926:c5350500-5350222 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T120397 NZ_CP037926:c5350500-5350222 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTACGGAGCTTCGAGACCTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11247.81 Da
• Isoelectric Point: 5.0747

>AT120397 WP_003113527.1 NZ_CP037926:c5350210-5349905 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMEFDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT120397 NZ_CP037926:c5350210-5349905 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTTGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCAAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Structures

Toxin

• AlphaFold DB: V6ALY3