Detailed information of TA system TA120395

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 4720087..4720768
• Replicon: chromosome
• Accession: NZ_CP037926
• Organism: Pseudomonas aeruginosa strain AES1R

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6AKP0
• Locus tag: CKAES1R_RS22785
• Protein ID: WP_003111825.1
• Coordinates: 4720403..4720768 (-)
• Length: 122 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CKAES1R_RS22780
• Protein ID: WP_003145733.1
• Coordinates: 4720087..4720410 (-)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CKAES1R_RS22755	4716001..4716639	+	639	WP_019681383.1	hypothetical protein	-
CKAES1R_RS22760	4716882..4717217	+	336	WP_003140884.1	TM2 domain-containing protein	-
CKAES1R_RS22765	4717391..4717908	+	518	Protein_4376	PAAR domain-containing protein	-
CKAES1R_RS22770	4717905..4718606	+	702	WP_019681384.1	VRR-NUC domain-containing protein	-
CKAES1R_RS22775	4718623..4719711	+	1089	WP_023088514.1	DUF3396 domain-containing protein	-
CKAES1R_RS22780	4720087..4720410	-	324	WP_003145733.1	XRE family transcriptional regulator	Antitoxin
CKAES1R_RS22785	4720403..4720768	-	366	WP_003111825.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CKAES1R_RS22790	4721059..4721298	-	240	WP_052156775.1	hypothetical protein	-
CKAES1R_RS22795	4721505..4721777	+	273	WP_019682407.1	hypothetical protein	-
CKAES1R_RS22800	4721808..4722233	-	426	WP_003085665.1	ring-cleaving dioxygenase	-
CKAES1R_RS22805	4722334..4723218	+	885	WP_019682408.1	LysR family transcriptional regulator	-
CKAES1R_RS22810	4723191..4724144	-	954	WP_003085661.1	LysR family transcriptional regulator	-
CKAES1R_RS22815	4724365..4724799	+	435	WP_003158601.1	RidA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	plcH	4675434..4754032	78598

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-63)

Sequences

Toxin

Protein

• Length: 122 a.a.
• Molecular weight: 13880.24 Da
• Isoelectric Point: 4.8219

>T120395 WP_003111825.1 NZ_CP037926:c4720768-4720403 [Pseudomonas aeruginosa]
VAWDIEYTDEFGDWWGSLSEDEQESLAVTVRLLEERGPSLGHPHSSGINGSRHGHMRELRTQHGGRPFRTLYAFDPRRSA
ILLIGGDKTGDDRWYELNVPIADRLYDEHLHQLREEGLIDG

Nucleotide

• Length: 366 bp

>T120395 NZ_CP037926:c4720768-4720403 [Pseudomonas aeruginosa]
GTGGCGTGGGATATCGAGTACACCGACGAGTTCGGTGATTGGTGGGGTTCTCTGTCCGAGGATGAGCAGGAGTCGCTAGC
TGTGACGGTTCGCTTGCTTGAGGAGCGAGGGCCATCCTTGGGGCACCCCCACAGCAGCGGCATCAATGGCTCCCGCCATG
GCCATATGAGAGAGCTACGGACTCAACACGGCGGGCGACCTTTCCGAACGCTTTACGCGTTTGACCCCAGACGCTCCGCG
ATACTGCTGATCGGTGGCGACAAGACTGGCGATGATCGTTGGTACGAATTGAACGTGCCCATTGCCGACCGCCTCTATGA
CGAACACTTGCACCAGCTCCGTGAGGAGGGACTGATCGATGGCTAA

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 11896.73 Da
• Isoelectric Point: 6.4751

>AT120395 WP_003145733.1 NZ_CP037926:c4720410-4720087 [Pseudomonas aeruginosa]
MAKKFSDLRAQMSPEAQARVEAKAQELLAEMPLNELRQARGLSQKMLSEVLHVQQPAIAKMERRTDMYISTLRSHIEAMG
GQLEVIARFPDGAVKISNFADIEEAGA

Nucleotide

• Length: 324 bp

>AT120395 NZ_CP037926:c4720410-4720087 [Pseudomonas aeruginosa]
ATGGCTAAGAAATTCTCCGACCTTCGGGCGCAAATGTCGCCTGAGGCACAGGCCCGCGTCGAGGCCAAGGCCCAGGAACT
GTTGGCTGAAATGCCCCTGAATGAGTTGCGCCAGGCTAGAGGCTTGTCGCAGAAGATGCTGTCTGAAGTGCTGCACGTGC
AGCAGCCGGCAATTGCCAAAATGGAGCGGCGTACGGATATGTACATCTCCACCTTGCGCAGCCACATCGAAGCCATGGGC
GGCCAGCTGGAAGTGATCGCACGCTTCCCGGATGGCGCGGTGAAAATCAGCAACTTCGCTGATATCGAAGAGGCGGGCGC
CTAA

Structures

Toxin

• AlphaFold DB: V6AKP0