Detailed information of TA system TA120387

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 1356772..1357453
• Replicon: chromosome
• Accession: NZ_CP037925
• Organism: Pseudomonas aeruginosa strain AES1M

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6AKP0
• Locus tag: CKAES1M_RS06445
• Protein ID: WP_003111825.1
• Coordinates: 1356772..1357137 (+)
• Length: 122 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CKAES1M_RS06450
• Protein ID: WP_003145733.1
• Coordinates: 1357130..1357453 (+)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CKAES1M_RS06415	1352741..1353175	-	435	WP_003158601.1	RidA family protein	-
CKAES1M_RS06420	1353396..1354349	+	954	WP_003085661.1	LysR family transcriptional regulator	-
CKAES1M_RS06425	1354322..1355206	-	885	WP_019682408.1	LysR family transcriptional regulator	-
CKAES1M_RS06430	1355307..1355732	+	426	WP_003085665.1	ring-cleaving dioxygenase	-
CKAES1M_RS06435	1355763..1356035	-	273	WP_019682407.1	hypothetical protein	-
CKAES1M_RS06440	1356242..1356481	+	240	WP_052156775.1	hypothetical protein	-
CKAES1M_RS06445	1356772..1357137	+	366	WP_003111825.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CKAES1M_RS06450	1357130..1357453	+	324	WP_003145733.1	XRE family transcriptional regulator	Antitoxin
CKAES1M_RS06455	1357829..1358917	-	1089	WP_023088514.1	DUF3396 domain-containing protein	-
CKAES1M_RS06460	1358934..1359635	-	702	WP_019681384.1	VRR-NUC domain-containing protein	-
CKAES1M_RS06465	1359632..1360150	-	519	WP_012614469.1	PAAR domain-containing protein	-
CKAES1M_RS06470	1360324..1360659	-	336	WP_003140884.1	TM2 domain-containing protein	-
CKAES1M_RS06475	1360902..1361540	-	639	WP_019681383.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	plcH	1338303..1423080	84777

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-63)

Sequences

Toxin

Protein

• Length: 122 a.a.
• Molecular weight: 13880.24 Da
• Isoelectric Point: 4.8219

>T120387 WP_003111825.1 NZ_CP037925:1356772-1357137 [Pseudomonas aeruginosa]
VAWDIEYTDEFGDWWGSLSEDEQESLAVTVRLLEERGPSLGHPHSSGINGSRHGHMRELRTQHGGRPFRTLYAFDPRRSA
ILLIGGDKTGDDRWYELNVPIADRLYDEHLHQLREEGLIDG

Nucleotide

• Length: 366 bp

>T120387 NZ_CP037925:1356772-1357137 [Pseudomonas aeruginosa]
GTGGCGTGGGATATCGAGTACACCGACGAGTTCGGTGATTGGTGGGGTTCTCTGTCCGAGGATGAGCAGGAGTCGCTAGC
TGTGACGGTTCGCTTGCTTGAGGAGCGAGGGCCATCCTTGGGGCACCCCCACAGCAGCGGCATCAATGGCTCCCGCCATG
GCCATATGAGAGAGCTACGGACTCAACACGGCGGGCGACCTTTCCGAACGCTTTACGCGTTTGACCCCAGACGCTCCGCG
ATACTGCTGATCGGTGGCGACAAGACTGGCGATGATCGTTGGTACGAATTGAACGTGCCCATTGCCGACCGCCTCTATGA
CGAACACTTGCACCAGCTCCGTGAGGAGGGACTGATCGATGGCTAA

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 11896.73 Da
• Isoelectric Point: 6.4751

>AT120387 WP_003145733.1 NZ_CP037925:1357130-1357453 [Pseudomonas aeruginosa]
MAKKFSDLRAQMSPEAQARVEAKAQELLAEMPLNELRQARGLSQKMLSEVLHVQQPAIAKMERRTDMYISTLRSHIEAMG
GQLEVIARFPDGAVKISNFADIEEAGA

Nucleotide

• Length: 324 bp

>AT120387 NZ_CP037925:1357130-1357453 [Pseudomonas aeruginosa]
ATGGCTAAGAAATTCTCCGACCTTCGGGCGCAAATGTCGCCTGAGGCACAGGCCCGCGTCGAGGCCAAGGCCCAGGAACT
GTTGGCTGAAATGCCCCTGAATGAGTTGCGCCAGGCTAGAGGCTTGTCGCAGAAGATGCTGTCTGAAGTGCTGCACGTGC
AGCAGCCGGCAATTGCCAAAATGGAGCGGCGTACGGATATGTACATCTCCACCTTGCGCAGCCACATCGAAGCCATGGGC
GGCCAGCTGGAAGTGATCGCACGCTTCCCGGATGGCGCGGTGAAAATCAGCAACTTCGCTGATATCGAAGAGGCGGGCGC
CTAA

Structures

Toxin

• AlphaFold DB: V6AKP0