Detailed information of TA system TA120385

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 727018..727613
• Replicon: chromosome
• Accession: NZ_CP037925
• Organism: Pseudomonas aeruginosa strain AES1M

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: CKAES1M_RS03510
• Protein ID: WP_003113526.1
• Coordinates: 727018..727296 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CKAES1M_RS03515
• Protein ID: WP_003113527.1
• Coordinates: 727308..727613 (+)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CKAES1M_RS03490	722444..723682	+	1239	WP_019681675.1	C69 family dipeptidase	-
CKAES1M_RS03495	723744..724391	+	648	WP_003095021.1	carbonate dehydratase	-
CKAES1M_RS03500	724461..726689	-	2229	WP_003113525.1	TonB-dependent receptor	-
CKAES1M_RS03510	727018..727296	+	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CKAES1M_RS03515	727308..727613	+	306	WP_003113527.1	HigA family addiction module antidote protein	Antitoxin
CKAES1M_RS03525	728019..729119	-	1101	WP_003099270.1	redox-regulated ATPase YchF	-
CKAES1M_RS03530	729160..729744	-	585	WP_003099278.1	aminoacyl-tRNA hydrolase	-
CKAES1M_RS03535	729786..730400	-	615	WP_003099279.1	50S ribosomal protein L25/general stress protein Ctc	-
CKAES1M_RS03540	730517..731458	-	942	WP_003099281.1	ribose-phosphate pyrophosphokinase	-
CKAES1M_RS03550	731625..732473	-	849	WP_003146058.1	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (22-69)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T120385 WP_003113526.1 NZ_CP037925:727018-727296 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T120385 NZ_CP037925:727018-727296 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTACGGAGCTTCGAGACCTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11247.81 Da
• Isoelectric Point: 5.0747

>AT120385 WP_003113527.1 NZ_CP037925:727308-727613 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMEFDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT120385 NZ_CP037925:727308-727613 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTTGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCAAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Structures

Toxin

• AlphaFold DB: V6ALY3