Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 2907536..2908230 Replicon chromosome
Accession NZ_CP037923
Organism Shigella flexneri 5a str. M90T

Toxin (Protein)


Gene name yafO Uniprot ID Q0T7Q5
Locus tag EKN05_RS15085 Protein ID WP_001263491.1
Coordinates 2907832..2908230 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID Q0T7Q4
Locus tag EKN05_RS15080 Protein ID WP_000554759.1
Coordinates 2907536..2907829 (+) Length 98 a.a.

Genomic Context

EKN05_RS15060EKN05_RS15070EKN05_RS15075EKN05_RS15080EKN05_RS15085EKN05_RS15090EKN05_RS15095EKN05_RS15100EKN05_RS15110EKN05_RS15065EKN05_RS15105
Location: 2903175..2903672 (498 bp)
Type: Others
Protein ID: WP_000006250.1
Location: 2905573..2906358 (786 bp)
Type: Others
Protein ID: WP_000207560.1
Location: 2906429..2907484 (1056 bp)
Type: Others
Protein ID: WP_001226172.1
Location: 2907536..2907829 (294 bp)
Type: Antitoxin
Protein ID: WP_000554759.1
Location: 2907832..2908230 (399 bp)
Type: Toxin
Protein ID: WP_001263491.1
Location: 2908240..2908692 (453 bp)
Type: Others
Protein ID: WP_001059862.1
Location: 2908938..2909981 (1044 bp)
Type: Others
Protein ID: WP_005060486.1
Location: 2910043..2910657 (615 bp)
Type: Others
Protein ID: Protein_2910
Location: 2912433..2912891 (459 bp)
Type: Others
Protein ID: WP_001291990.1
Location: 2903889..2905601 (1713 bp)
Type: Others
Protein ID: Protein_2903
Location: 2910714..2912171 (1458 bp)
Type: Others
Protein ID: WP_001293006.1
ToxinAntitoxinOther genes
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Locus tag
Coordinates
Strand
Size (bp)
Protein ID
Product
Description
EKN05_RS15060 (EKN05_015075)2903175..2903672+498WP_000006250.1transposase-
EKN05_RS15065 (EKN05_015080)2903889..2905601-1713Protein_2903flagellar biosynthesis protein FlhA-
EKN05_RS15070 (EKN05_015085)2905573..2906358+786WP_000207560.1putative lateral flagellar export/assembly protein LafU-
EKN05_RS15075 (EKN05_015090)2906429..2907484+1056WP_001226172.1DNA polymerase IV-
EKN05_RS15080 (EKN05_015095)2907536..2907829+294WP_000554759.1type I toxin-antitoxin system antitoxin YafNAntitoxin
EKN05_RS15085 (EKN05_015100)2907832..2908230+399WP_001263491.1type II toxin-antitoxin system mRNA interferase toxin YafOToxin
EKN05_RS15090 (EKN05_015105)2908240..2908692+453WP_001059862.1GNAT family N-acetyltransferase-
EKN05_RS15095 (EKN05_015110)2908938..2909981+1044WP_005060486.1RNA ligase RtcB family protein-
EKN05_RS15100 (EKN05_015115)2910043..2910657+615Protein_2910peptide chain release factor H-
EKN05_RS15105 (EKN05_015120)2910714..2912171-1458WP_001293006.1cytosol nonspecific dipeptidase-
EKN05_RS15110 (EKN05_015125)2912433..2912891+459WP_001291990.1xanthine phosphoribosyltransferase-

Associated MGEs

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MGE
detail
Similar
MGEs
Relative
position
MGE Type
Cargo ARG
Virulence gene
Coordinates
Length (bp)
No matching records found


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15457.86 Da        Isoelectric Point: 8.0949

>T120377 WP_001263491.1 NZ_CP037923:2907832-2908230 [Shigella flexneri 5a str. M90T]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T120377 NZ_CP037923:2907832-2908230 [Shigella flexneri 5a str. M90T]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAGCCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGAAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11232.82 Da        Isoelectric Point: 7.0153

>AT120377 WP_000554759.1 NZ_CP037923:2907536-2907829 [Shigella flexneri 5a str. M90T]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
KQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT120377 NZ_CP037923:2907536-2907829 [Shigella flexneri 5a str. M90T]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
AGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTTCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
AAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

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Protein
Organism
Identities (%)
Coverage (%)
Ha-value
No matching records found
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Protein
Organism
Identities (%)
Coverage (%)
Ha-value
No matching records found

Structures

Toxin

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Source
ID
Structure
AlphaFold DBA0A4P7TPK7

Antitoxin

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Source
ID
Structure
AlphaFold DBA0A4P7TR46

References