Detailed information of TA system TA120360

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 356711..357438
• Replicon: chromosome
• Accession: NZ_CP037923
• Organism: Shigella flexneri 5a str. M90T

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A4P7TJ48
• Locus tag: EKN05_RS01780
• Protein ID: WP_000547561.1
• Coordinates: 357127..357438 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: EKN05_RS01775
• Protein ID: WP_000126296.1
• Coordinates: 356711..357130 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EKN05_RS01755 (EKN05_001765)	351760..352287	-	528	WP_001078777.1	electron transport protein HydN	-
EKN05_RS01760 (EKN05_001770)	352437..353447	-	1011	WP_005051459.1	DNA-binding transcriptional regulator AscG	-
EKN05_RS01765 (EKN05_001775)	353697..355163	+	1467	WP_136350408.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
EKN05_RS01770 (EKN05_001780)	355172..356596	+	1425	WP_005105741.1	6-phospho-beta-glucosidase AscB	-
EKN05_RS01775 (EKN05_001785)	356711..357130	-	420	WP_000126296.1	helix-turn-helix domain-containing protein	Antitoxin
EKN05_RS01780 (EKN05_001790)	357127..357438	-	312	WP_000547561.1	type II toxin-antitoxin system HigB family toxin	Toxin
EKN05_RS01785 (EKN05_001795)	357611..358354	-	744	WP_044024082.1	hypothetical protein	-
EKN05_RS01790 (EKN05_001800)	358397..358867	-	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
EKN05_RS01795 (EKN05_001805)	358860..359270	-	411	WP_001291916.1	formate hydrogenlyase assembly protein HycH	-
EKN05_RS01800 (EKN05_001810)	359267..360034	-	768	WP_000067379.1	formate hydrogenlyase subunit HycG	-
EKN05_RS01805 (EKN05_001815)	360034..360576	-	543	WP_000493785.1	formate hydrogenlyase subunit HycF	-
EKN05_RS01810 (EKN05_001820)	360586..362295	-	1710	WP_005063855.1	formate hydrogenlyase subunit HycE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12370.06 Da
• Isoelectric Point: 9.7105

>T120360 WP_000547561.1 NZ_CP037923:c357438-357127 [Shigella flexneri 5a str. M90T]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDAGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T120360 NZ_CP037923:c357438-357127 [Shigella flexneri 5a str. M90T]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTCTGCGCGTAAATATCCTAACGACGCTCTGGCTCTTCAGGCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACGCCAGAAGAGATGCGAACGGCGTTTCCCAACCTCGACAATTTTAAATATC
GCAACAAATGGTACGTTCTGGATGCAGGTGGGAACAACCTAAGGGTGATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACTAATCACGCCGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.38 Da
• Isoelectric Point: 4.3653

>AT120360 WP_000126296.1 NZ_CP037923:c357130-356711 [Shigella flexneri 5a str. M90T]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNQEFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT120360 NZ_CP037923:c357130-356711 [Shigella flexneri 5a str. M90T]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGTAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAATATGAAAACAACAACCAAGAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCTGTT
GGCGTGGCGTTACTTCGCACGCTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGTCAGATTTTGTCTGGACAACGATCCCTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: A0A4P7TJ48