Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 3530385..3531042 Replicon chromosome
Accession NZ_CP037919
Organism Enterobacter cloacae complex sp. strain N13-01531

Toxin (Protein)


Gene name higB Uniprot ID A0A1Z3MZ87
Locus tag E2E36_RS16785 Protein ID WP_063408809.1
Coordinates 3530385..3530684 (+) Length 100 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A1Z3MZ32
Locus tag E2E36_RS16790 Protein ID WP_063408808.1
Coordinates 3530695..3531042 (+) Length 116 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E2E36_RS16775 3526756..3528954 + 2199 WP_133295003.1 type I secretion system permease/ATPase -
E2E36_RS16780 3528965..3530134 + 1170 WP_086374946.1 HlyD family efflux transporter periplasmic adaptor subunit -
E2E36_RS16785 3530385..3530684 + 300 WP_063408809.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
E2E36_RS16790 3530695..3531042 + 348 WP_063408808.1 HigA family addiction module antidote protein Antitoxin
E2E36_RS16795 3531063..3531581 - 519 WP_133295004.1 hypothetical protein -
E2E36_RS16800 3531667..3532848 - 1182 WP_133295005.1 PLP-dependent aminotransferase family protein -
E2E36_RS16805 3532868..3533755 - 888 WP_133295006.1 LysR family transcriptional regulator -
E2E36_RS16810 3533853..3534455 + 603 WP_023308908.1 short chain dehydrogenase -
E2E36_RS16815 3534572..3535951 - 1380 WP_133295007.1 amidohydrolase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - - 3430512..3538709 108197


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(1-95)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(8-86)


Sequences

Toxin        


Download         Length: 100 a.a.        Molecular weight: 11707.32 Da        Isoelectric Point: 10.0346

>T120356 WP_063408809.1 NZ_CP037919:3530385-3530684 [Enterobacter cloacae complex sp.]
MISYFRDQWLEDFFLYGRSSNVIPSNLETALARKLDIIRAATSHRDLRSPPGNMYEALNPPLKGYSSIRVNRQYRLVFRW
TEGKAEDLYLSPHKYTQHK

Download         Length: 300 bp

>T120356 NZ_CP037919:3530385-3530684 [Enterobacter cloacae complex sp.]
ATGATTAGTTATTTTCGGGATCAATGGCTTGAGGATTTTTTTCTTTACGGCAGATCGAGCAATGTTATCCCTTCAAATCT
GGAAACAGCGCTTGCGAGAAAGCTCGATATCATCAGGGCAGCAACCTCGCACAGGGATTTGCGATCGCCACCGGGTAATA
TGTATGAAGCATTGAATCCTCCGCTGAAGGGCTATTCCTCCATCCGGGTAAACAGGCAATACAGGCTCGTATTTCGCTGG
ACAGAGGGTAAAGCAGAAGATCTGTACCTCTCTCCACACAAGTACACGCAACACAAGTGA

Antitoxin


Download         Length: 116 a.a.        Molecular weight: 13039.98 Da        Isoelectric Point: 7.0134

>AT120356 WP_063408808.1 NZ_CP037919:3530695-3531042 [Enterobacter cloacae complex sp.]
MTLQQALRKPTTPGDVLQYEYLEPLNLKISDLAEMLNVHRNTISALVNNNRKLTADMAIKLAKAFDTTIEFWLNLQLNVD
IWEAQSNARTQEELSRIKTVADVMAKRKSGQPDVA

Download         Length: 348 bp

>AT120356 NZ_CP037919:3530695-3531042 [Enterobacter cloacae complex sp.]
ATGACACTTCAACAGGCACTCCGCAAACCCACCACGCCGGGCGACGTGTTGCAGTATGAGTATCTTGAACCGCTCAATCT
GAAAATTAGCGATCTGGCGGAGATGCTCAATGTTCACCGCAACACCATCAGCGCGCTGGTCAATAACAATCGCAAGCTTA
CTGCCGATATGGCGATCAAGCTGGCAAAAGCCTTTGATACCACGATTGAATTCTGGCTGAACTTACAGCTGAACGTTGAT
ATCTGGGAAGCGCAATCTAACGCCAGGACGCAGGAGGAACTAAGCCGTATAAAGACCGTTGCGGACGTCATGGCTAAGCG
AAAATCTGGCCAACCGGATGTAGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Z3MZ87

Antitoxin

Source ID Structure
AlphaFold DB A0A1Z3MZ32

References