Detailed information of TA system TA120338

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 75373..75643
• Replicon: plasmid pYSP8-1-CTX-M-14
• Accession: NZ_CP037912
• Organism: Escherichia coli strain YSP8-1

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: E1J56_RS25055
• Protein ID: WP_001312861.1
• Coordinates: 75485..75643 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	75373..75436 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E1J56_RS25030	71084..71611	+	528	WP_000290834.1	single-stranded DNA-binding protein	-
E1J56_RS25035	71669..71902	+	234	WP_000006003.1	DUF905 family protein	-
E1J56_RS25040	71963..73986	+	2024	Protein_77	ParB/RepB/Spo0J family partition protein	-
E1J56_RS25045	74055..74489	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
E1J56_RS25050	74486..75205	+	720	WP_001276217.1	plasmid SOS inhibition protein A	-
-	75217..75441	+	225	NuclAT_0	-	-
-	75217..75441	+	225	NuclAT_0	-	-
-	75217..75441	+	225	NuclAT_0	-	-
-	75217..75441	+	225	NuclAT_0	-	-
E1J56_RS25695	75226..75405	-	180	WP_001309233.1	hypothetical protein	-
-	75373..75436	-	64	-	-	Antitoxin
E1J56_RS25055	75485..75643	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
E1J56_RS25815	75881..76258	-	378	Protein_82	hypothetical protein	-
E1J56_RS25075	76558..76854	+	297	WP_001272251.1	hypothetical protein	-
E1J56_RS25080	76965..77786	+	822	WP_001234445.1	DUF945 domain-containing protein	-
E1J56_RS25085	78083..78685	-	603	WP_000243713.1	transglycosylase SLT domain-containing protein	-
E1J56_RS25095	79008..79391	+	384	WP_001354030.1	relaxosome protein TraM	-
E1J56_RS25100	79585..80256	+	672	WP_000283561.1	conjugal transfer transcriptional regulator TraJ	-
E1J56_RS25105	80393..80620	+	228	WP_000089263.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sul2 / erm(42) / ant(3'')-Ia / sitABCD / blaCTX-M-14 / qacE / cmlA1 / tet(M)	iucA / iucB / iucC / iucD / iutA	1..149304	149304

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T120338 WP_001312861.1 NZ_CP037912:75485-75643 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T120338 NZ_CP037912:75485-75643 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 64 bp

>AT120338 NZ_CP037912:c75436-75373 [Escherichia coli]
GACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCTT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin