Detailed information of TA system TA120309

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 1977..2623
• Replicon: plasmid unnamed4
• Accession: NZ_CP037908
• Organism: Escherichia coli strain LHM10-1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A3P1YQM6
• Locus tag: E1J55_RS24995
• Protein ID: WP_025667955.1
• Coordinates: 2276..2623 (-)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: E1J55_RS24990
• Protein ID: WP_112920252.1
• Coordinates: 1977..2276 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E1J55_RS24980	674..1378	-	705	WP_001067858.1	IS6-like element IS26 family transposase	-
E1J55_RS24985	1659..1943	+	285	WP_024227107.1	hypothetical protein	-
E1J55_RS24990	1977..2276	-	300	WP_112920252.1	XRE family transcriptional regulator	Antitoxin
E1J55_RS24995	2276..2623	-	348	WP_025667955.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
E1J55_RS25000	2988..3635	+	648	WP_001615566.1	ParA family protein	-
E1J55_RS25005	3675..3908	+	234	WP_001615564.1	hypothetical protein	-
E1J55_RS25010	3947..4483	-	537	WP_112920253.1	hypothetical protein	-
E1J55_RS25015	4493..4957	-	465	WP_112920254.1	thermonuclease family protein	-
E1J55_RS25020	4961..5695	-	735	WP_112920255.1	molybdopterin-guanine dinucleotide biosynthesis protein MobC	-
E1J55_RS25025	5707..7434	-	1728	WP_001350940.1	type IV secretory system conjugative DNA transfer family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..28519	28519
	-	flank	IS/Tn	-	-	674..1378	704

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-105)

Antitoxin

• Cro/C1-type helix-turn-helix domain (32-80)

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 13530.50 Da
• Isoelectric Point: 9.3634

>T120309 WP_025667955.1 NZ_CP037908:c2623-2276 [Escherichia coli]
MWTVLFSQRFDDWLNEQEDALQEKVLADLKNLQVYGPELPRPYADTVKGSRYKNMKELRVQFSGRPIRAFYAFDPIRRAI
VLCAGDKSNDKRFYKKLVRIAEDEFAAHLNTLESK

Nucleotide

• Length: 348 bp

>T120309 NZ_CP037908:c2623-2276 [Escherichia coli]
ATGTGGACGGTACTTTTTAGCCAGCGATTTGACGACTGGCTAAATGAGCAAGAAGACGCTCTTCAGGAAAAGGTTTTGGC
TGATTTGAAAAATCTACAGGTTTACGGACCTGAGTTACCCAGGCCATATGCCGATACCGTAAAAGGTTCTCGGTACAAAA
ATATGAAAGAGCTTCGCGTTCAGTTTTCTGGCCGTCCGATAAGAGCCTTTTATGCGTTCGATCCGATTCGCCGGGCTATC
GTTCTTTGTGCAGGAGACAAAAGTAACGATAAGCGGTTTTATAAAAAACTGGTGCGTATAGCTGAGGATGAGTTTGCAGC
ACATCTGAACACACTGGAGAGCAAGTAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11083.93 Da
• Isoelectric Point: 5.8962

>AT120309 WP_112920252.1 NZ_CP037908:c2276-1977 [Escherichia coli]
MRTLEEVIASRSPESQARIKEMADEMILEVGLQMMREELQLSQKQVAEAMGISQPAVTKLEQRGNDLKLATLKRYVEAMG
GKLSLDVELPTGKRVAFHV

Nucleotide

• Length: 300 bp

>AT120309 NZ_CP037908:c2276-1977 [Escherichia coli]
ATGAGAACATTAGAAGAGGTGATTGCCAGCCGTTCACCGGAAAGCCAGGCGCGAATTAAAGAAATGGCAGATGAGATGAT
TCTTGAGGTCGGCTTGCAGATGATGCGTGAAGAACTCCAGTTATCACAAAAACAGGTTGCTGAGGCGATGGGTATTAGTC
AGCCAGCAGTAACAAAGCTGGAGCAGCGCGGAAATGATTTAAAGCTGGCGACATTAAAGCGTTACGTTGAAGCTATGGGA
GGCAAATTAAGCCTGGATGTTGAGCTTCCTACAGGAAAGCGAGTAGCGTTCCATGTCTAG

Structures

Toxin

• AlphaFold DB: A0A3P1YQM6