Detailed information of TA system TA120302

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 76914..77340
• Replicon: plasmid unnamed1
• Accession: NZ_CP037904
• Organism: Escherichia coli strain LHM10-1

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: E1J55_RS23730
• Protein ID: WP_001312861.1
• Coordinates: 76914..77072 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	77116..77340 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E1J55_RS23690	72283..72972	-	690	WP_039002187.1	conjugal transfer transcriptional regulator TraJ	-
E1J55_RS23695	73159..73542	-	384	WP_001151524.1	relaxosome protein TraM	-
E1J55_RS23700	73863..74465	+	603	WP_000243712.1	transglycosylase SLT domain-containing protein	-
E1J55_RS23705	74762..75583	-	822	WP_072795862.1	DUF945 domain-containing protein	-
E1J55_RS23710	75705..75992	-	288	WP_000107535.1	hypothetical protein	-
E1J55_RS23730	76914..77072	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	77116..77340	-	225	NuclAT_0	-	Antitoxin
-	77116..77340	-	225	NuclAT_0	-	Antitoxin
-	77116..77340	-	225	NuclAT_0	-	Antitoxin
-	77116..77340	-	225	NuclAT_0	-	Antitoxin
E1J55_RS25410	77152..77340	+	189	WP_001299721.1	hypothetical protein	-
E1J55_RS23735	77352..78071	-	720	WP_001276232.1	plasmid SOS inhibition protein A	-
E1J55_RS23740	78068..78502	-	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
E1J55_RS23745	78557..80515	-	1959	WP_039000998.1	ParB/RepB/Spo0J family partition protein	-
E1J55_RS23750	80580..80813	-	234	WP_000005987.1	DUF905 family protein	-
E1J55_RS23755	80870..81409	-	540	WP_032332913.1	single-stranded DNA-binding protein	-
E1J55_RS25490	81435..81641	-	207	WP_000275853.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	mph(A) / tet(M) / qnrS1 / floR / ant(3'')-Ia / aac(3)-VIa	htpB	1..108366	108366

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T120302 WP_001312861.1 NZ_CP037904:c77072-76914 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T120302 NZ_CP037904:c77072-76914 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT120302 NZ_CP037904:c77340-77116 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin