Detailed information of TA system TA120298

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 41624..41878
• Replicon: plasmid unnamed1
• Accession: NZ_CP037904
• Organism: Escherichia coli strain LHM10-1

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: E1J55_RS23530
• Protein ID: WP_001351576.1
• Coordinates: 41624..41830 (-)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	41817..41878 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E1J55_RS23485	37941..38645	+	705	WP_001067858.1	IS6-like element IS26 family transposase	-
E1J55_RS23495	38776..38994	+	219	Protein_42	IS1 family transposase	-
E1J55_RS25405	39026..39241	-	216	WP_061583825.1	hypothetical protein	-
E1J55_RS23510	39943..40800	-	858	WP_039002220.1	incFII family plasmid replication initiator RepA	-
E1J55_RS23515	40793..40867	-	75	WP_061357229.1	RepA leader peptide Tap	-
E1J55_RS23525	41099..41356	-	258	WP_001541895.1	replication regulatory protein RepA	-
E1J55_RS23530	41624..41830	-	207	WP_001351576.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	41817..41878	+	62	NuclAT_1	-	Antitoxin
-	41817..41878	+	62	NuclAT_1	-	Antitoxin
-	41817..41878	+	62	NuclAT_1	-	Antitoxin
-	41817..41878	+	62	NuclAT_1	-	Antitoxin
E1J55_RS23540	42220..42432	-	213	WP_001541896.1	ANR family transcriptional regulator	-
E1J55_RS23545	42567..43127	-	561	WP_000139310.1	fertility inhibition protein FinO	-
E1J55_RS23550	43182..43925	-	744	WP_039002216.1	conjugal transfer pilus acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	mph(A) / tet(M) / qnrS1 / floR / ant(3'')-Ia / aac(3)-VIa	htpB	1..108366	108366

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7826.31 Da
• Isoelectric Point: 8.8807

>T120298 WP_001351576.1 NZ_CP037904:c41830-41624 [Escherichia coli]
MKYLNTTDCSLFFAERSKFMTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 207 bp

>T120298 NZ_CP037904:c41830-41624 [Escherichia coli]
ATGAAGTACCTTAACACTACTGATTGTAGCCTCTTCTTTGCAGAGAGGTCAAAGTTTATGACGAAATATGCCCTTATCGG
GTTGCTCGCCGTGTGCGCCACTGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTTATGTGAGCTGAATATTCACAGAG
GAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 62 bp

>AT120298 NZ_CP037904:41817-41878 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin