Detailed information of TA system TA120297

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4609482..4610084
• Replicon: chromosome
• Accession: NZ_CP037903
• Organism: Escherichia coli strain LHM10-1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: E1J55_RS22315
• Protein ID: WP_000897305.1
• Coordinates: 4609773..4610084 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: E1J55_RS22310
• Protein ID: WP_000356397.1
• Coordinates: 4609482..4609772 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E1J55_RS22285	4605410..4606312	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
E1J55_RS22290	4606309..4606944	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
E1J55_RS22295	4606941..4607870	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
E1J55_RS22300	4608199..4608441	-	243	WP_024199136.1	ribbon-helix-helix domain-containing protein	-
E1J55_RS22305	4608660..4608878	-	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
E1J55_RS22310	4609482..4609772	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
E1J55_RS22315	4609773..4610084	-	312	WP_000897305.1	hypothetical protein	Toxin
E1J55_RS22320	4610313..4611221	+	909	WP_136698623.1	alpha/beta hydrolase	-
E1J55_RS22325	4611390..4613204	-	1815	WP_112914494.1	hypothetical protein	-
E1J55_RS22330	4613619..4614560	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
E1J55_RS22335	4614605..4615042	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T120297 WP_000897305.1 NZ_CP037903:c4610084-4609773 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T120297 NZ_CP037903:c4610084-4609773 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT120297 WP_000356397.1 NZ_CP037903:c4609772-4609482 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT120297 NZ_CP037903:c4609772-4609482 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAAGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTACTG
CGAGCGATTGCCAATGATCCGCAACATGTACTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7