Detailed information of TA system TA120278

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-YefM
• Location: 1850758..1851260
• Replicon: chromosome
• Accession: NZ_CP037903
• Organism: Escherichia coli strain LHM10-1

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: -
• Locus tag: E1J55_RS08780
• Protein ID: WP_000767835.1
• Coordinates: 1851006..1851260 (+)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: S1Q063
• Locus tag: E1J55_RS08775
• Protein ID: WP_001259255.1
• Coordinates: 1850758..1851009 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E1J55_RS08750	1845935..1847002	-	1068	WP_000080072.1	bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB	-
E1J55_RS08755	1847002..1848072	-	1071	WP_000108941.1	histidinol-phosphate transaminase	-
E1J55_RS08760	1848069..1849373	-	1305	WP_001413482.1	histidinol dehydrogenase	-
E1J55_RS08765	1849379..1850278	-	900	WP_000131774.1	ATP phosphoribosyltransferase	-
E1J55_RS08770	1850424..1850474	-	51	WP_001364200.1	his operon leader peptide	-
E1J55_RS08775	1850758..1851009	+	252	WP_001259255.1	YoeB-YefM toxin-antitoxin system antitoxin YefM	Antitoxin
E1J55_RS08780	1851006..1851260	+	255	WP_000767835.1	type II toxin-antitoxin system mRNA interferase toxin YoeB	Toxin
E1J55_RS08785	1851343..1852167	+	825	WP_000754737.1	SDR family oxidoreductase	-
E1J55_RS08790	1852213..1853142	+	930	WP_000803351.1	LysR family transcriptional regulator	-
E1J55_RS08795	1853357..1853419	+	63	WP_010723108.1	membrane protein YoeI	-
E1J55_RS08800	1853409..1854767	+	1359	WP_000019197.1	putrescine/proton symporter PlaP	-
E1J55_RS08805	1854946..1856004	+	1059	WP_000492321.1	transport protein YeeE	-
E1J55_RS08810	1856018..1856245	+	228	WP_000985273.1	sulfurtransferase TusA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (2-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10169.51 Da
• Isoelectric Point: 7.2751

>T120278 WP_000767835.1 NZ_CP037903:1851006-1851260 [Escherichia coli]
VKLIWSEESWDDYLYWQETDNRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Nucleotide

• Length: 255 bp

>T120278 NZ_CP037903:1851006-1851260 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAGTCATGGGATGATTATCTGTACTGGCAGGAAACAGATAATCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAGCATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9307.58 Da
• Isoelectric Point: 4.7954

>AT120278 WP_001259255.1 NZ_CP037903:1850758-1851009 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Nucleotide

• Length: 252 bp

>AT120278 NZ_CP037903:1850758-1851009 [Escherichia coli]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Structures

Antitoxin

• AlphaFold DB: A0A7U9LNR0