Detailed information of TA system TA120253

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2564683..2565245
• Replicon: chromosome
• Accession: NZ_CP037864
• Organism: Citrobacter sp. LY-1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: E1B03_RS13385
• Protein ID: WP_133087223.1
• Coordinates: 2564683..2564961 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: E1B03_RS13390
• Protein ID: WP_103770609.1
• Coordinates: 2564961..2565245 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E1B03_RS13375	2561379..2562281	-	903	WP_103770606.1	endonuclease/exonuclease/phosphatase family protein	-
E1B03_RS13380	2562748..2564463	+	1716	WP_133086375.1	ATP-binding cassette domain-containing protein	-
E1B03_RS13385	2564683..2564961	+	279	WP_133087223.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
E1B03_RS13390	2564961..2565245	+	285	WP_103770609.1	HigA family addiction module antidote protein	Antitoxin
E1B03_RS13395	2565289..2565891	-	603	WP_103770610.1	inorganic diphosphatase	-
E1B03_RS13400	2566021..2566677	-	657	WP_016153106.1	formate dehydrogenase-N subunit gamma	-
E1B03_RS13405	2566670..2567554	-	885	WP_003833024.1	formate dehydrogenase N subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (3-81)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10712.37 Da
• Isoelectric Point: 10.1795

>T120253 WP_133087223.1 NZ_CP037864:2564683-2564961 [Citrobacter sp. LY-1]
MIMNFRHKGLRDLFLHGRTSGVMAKHVRRLRHRLAVIDAASKVTDINMPGYKLHPLMGDRDGIWAIAVSGNWRITFEFVN
GDAYIRDYEDYH

Nucleotide

• Length: 279 bp

>T120253 NZ_CP037864:2564683-2564961 [Citrobacter sp. LY-1]
ATGATCATGAACTTCAGACACAAGGGATTGCGCGATTTGTTTCTTCATGGACGAACATCAGGCGTTATGGCGAAACATGT
CCGACGATTACGACACAGGCTTGCAGTTATTGATGCTGCAAGCAAGGTTACAGATATCAATATGCCCGGCTATAAGTTAC
ATCCTCTGATGGGTGATCGTGACGGAATATGGGCAATTGCCGTTTCAGGTAACTGGCGTATTACGTTTGAATTCGTCAAT
GGCGACGCGTATATACGGGACTACGAGGACTATCATTAA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10357.03 Da
• Isoelectric Point: 8.8567

>AT120253 WP_103770609.1 NZ_CP037864:2564961-2565245 [Citrobacter sp. LY-1]
MKMANHPRPGDIIQEALDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPEMWLNLQNTWSLAEAQ
KSVDISRLRRLAAQ

Nucleotide

• Length: 285 bp

>AT120253 NZ_CP037864:2564961-2565245 [Citrobacter sp. LY-1]
ATGAAGATGGCAAATCATCCCCGTCCTGGGGATATCATTCAGGAAGCGCTGGACGAACTCAATGTCAGCCTGCGCGAGTT
TGCCAGAGCTATGGAGATTGCCCCTTCAACGGCAAGCCGACTATTAACCGGGAAAGCGGCCCTTACGCCAGAGATGGCGA
TTAAACTCTCCGTGGTGATTGGCAGTTCACCTGAAATGTGGTTAAACCTGCAAAATACCTGGAGCCTGGCAGAAGCCCAA
AAAAGCGTGGATATTTCACGCCTGCGTAGGCTGGCAGCGCAGTGA