Detailed information of TA system TA120244

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2101741..2102459
• Replicon: chromosome
• Accession: NZ_CP037861
• Organism: Pasteurella multocida subsp. multocida strain HN01

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: E0L18_RS10565
• Protein ID: WP_139617699.1
• Coordinates: 2102133..2102459 (-)
• Length: 109 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: E0L18_RS10560
• Protein ID: WP_005757699.1
• Coordinates: 2101741..2102067 (-)
• Length: 109 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E0L18_RS10540	2097791..2098249	+	459	Protein_2039	hypothetical protein	-
E0L18_RS10545	2098485..2099468	+	984	WP_139617696.1	hypothetical protein	-
E0L18_RS10550	2099512..2100108	+	597	WP_139617697.1	16S rRNA (guanine(966)-N(2))-methyltransferase RsmD	-
E0L18_RS10555	2100167..2101687	+	1521	WP_139617698.1	DUF560 domain-containing protein	-
E0L18_RS10560	2101741..2102067	-	327	WP_005757699.1	HigA family addiction module antidote protein	Antitoxin
E0L18_RS10565	2102133..2102459	-	327	WP_139617699.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
E0L18_RS10570	2102617..2103093	+	477	WP_139617700.1	tRNA (uridine(34)/cytosine(34)/5- carboxymethylaminomethyluridine(34)-2'-O)- methyltransferase TrmL	-
E0L18_RS10575	2103168..2104037	-	870	WP_139617701.1	VirK/YbjX family protein	-
E0L18_RS10580	2104148..2104765	+	618	WP_005718080.1	ribosome biogenesis GTP-binding protein YihA/YsxC	-
E0L18_RS10585	2104873..2105745	+	873	WP_139617702.1	YifB family Mg chelatase-like AAA ATPase	-
E0L18_RS10590	2105858..2106184	-	327	WP_139617703.1	helix-turn-helix domain-containing protein	-
E0L18_RS10595	2106232..2107038	+	807	Protein_2050	recombinase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (12-104)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-102)

Sequences

Toxin

Protein

• Length: 109 a.a.
• Molecular weight: 12878.71 Da
• Isoelectric Point: 9.6705

>T120244 WP_139617699.1 NZ_CP037861:c2102459-2102133 [Pasteurella multocida subsp. multocida]
MDRVRFNLTEDSFQDKYLYEYFLYGSKHKNIPSDIENTLAHKLDMLDSANTLSDLRSPPGNKLEKLDPKTSGLYSIRVNV
KYRLIFKYKESNFPKITELRLDKHQYRL

Nucleotide

• Length: 327 bp

>T120244 NZ_CP037861:c2102459-2102133 [Pasteurella multocida subsp. multocida]
ATGGACAGAGTCCGATTTAATCTTACGGAAGATAGCTTCCAAGACAAGTATCTGTATGAGTATTTTTTATATGGAAGTAA
GCATAAAAATATTCCAAGTGATATAGAAAATACGCTAGCGCATAAATTAGATATGTTAGATAGCGCAAATACTTTGAGTG
ATTTACGAAGTCCACCTGGTAATAAATTGGAAAAATTGGACCCTAAAACGAGTGGGCTATATTCTATCAGAGTGAATGTG
AAGTATCGTTTAATATTTAAATATAAAGAAAGTAATTTTCCAAAAATAACAGAGTTGAGATTAGATAAACATCAATATAG
ATTATAA

Antitoxin

Protein

• Length: 109 a.a.
• Molecular weight: 12377.41 Da
• Isoelectric Point: 7.2034

>AT120244 WP_005757699.1 NZ_CP037861:c2102067-2101741 [Pasteurella multocida subsp. multocida]
MSRVQRKPSTVGDILLDEYLVPLNLKIADLATMLDVHRNTASALVNNNTKLSLEMALKLAKLFNTSPEFWLNLQMKLDLW
EIENNARFQESLKKISSIDQHNLLEKIA

Nucleotide

• Length: 327 bp

>AT120244 NZ_CP037861:c2102067-2101741 [Pasteurella multocida subsp. multocida]
ATGAGTAGAGTACAAAGAAAGCCATCAACAGTGGGTGATATTTTATTAGATGAATATTTAGTTCCACTAAATTTGAAAAT
TGCAGATTTAGCTACAATGTTAGATGTTCATCGTAATACAGCAAGTGCACTCGTTAATAATAATACTAAATTGTCTTTAG
AAATGGCACTGAAACTAGCAAAACTATTTAATACAAGTCCTGAGTTTTGGTTAAATTTACAAATGAAATTAGACTTATGG
GAAATTGAAAACAATGCTAGGTTTCAAGAAAGTTTAAAGAAAATTTCGTCTATTGATCAGCATAATCTGTTAGAAAAAAT
CGCTTGA