Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2082354..2082856 Replicon chromosome
Accession NZ_CP037857
Organism Escherichia coli BW25113 strain AM187

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag E1A98_RS10415 Protein ID WP_000767829.1
Coordinates 2082354..2082608 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag E1A98_RS10420 Protein ID WP_001259255.1
Coordinates 2082605..2082856 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E1A98_RS10385 2077369..2077596 - 228 WP_000234896.1 sulfurtransferase TusA -
E1A98_RS10390 2077610..2078668 - 1059 WP_000492339.1 transport protein YeeE -
E1A98_RS10395 2078847..2080205 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
E1A98_RS10400 2080195..2080257 - 63 WP_010723108.1 membrane protein YoeI -
E1A98_RS10405 2080472..2081401 - 930 WP_000803351.1 LysR family transcriptional regulator -
E1A98_RS10410 2081447..2082271 - 825 WP_000754737.1 SDR family oxidoreductase -
E1A98_RS10415 2082354..2082608 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
E1A98_RS10420 2082605..2082856 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
E1A98_RS10425 2083139..2083189 + 51 WP_001364200.1 his operon leader peptide -
E1A98_RS10430 2083335..2084234 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
E1A98_RS10435 2084240..2085544 + 1305 WP_000009594.1 histidinol dehydrogenase -
E1A98_RS10440 2085541..2086611 + 1071 WP_000108941.1 histidinol-phosphate transaminase -
E1A98_RS10445 2086611..2087678 + 1068 WP_000080105.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T120223 WP_000767829.1 NZ_CP037857:c2082608-2082354 [Escherichia coli BW25113]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T120223 NZ_CP037857:c2082608-2082354 [Escherichia coli BW25113]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT120223 WP_001259255.1 NZ_CP037857:c2082856-2082605 [Escherichia coli BW25113]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT120223 NZ_CP037857:c2082856-2082605 [Escherichia coli BW25113]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References