Detailed information of TA system TA120003

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 553489..554095
• Replicon: chromosome
• Accession: NZ_CP037745
• Organism: Rheinheimera sp. D18

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: E0Z06_RS02650
• Protein ID: WP_207903173.1
• Coordinates: 553778..554095 (-)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: E0Z06_RS02645
• Protein ID: WP_132581636.1
• Coordinates: 553489..553776 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E0Z06_RS02625	548564..549172	-	609	WP_132581628.1	dephospho-CoA kinase	-
E0Z06_RS02630	549189..550067	-	879	WP_132581630.1	A24 family peptidase	-
E0Z06_RS02635	550067..551296	-	1230	WP_132581632.1	type II secretion system F family protein	-
E0Z06_RS02640	551377..553068	-	1692	WP_132581634.1	type IV-A pilus assembly ATPase PilB	-
E0Z06_RS02645	553489..553776	-	288	WP_132581636.1	helix-turn-helix domain-containing protein	Antitoxin
E0Z06_RS02650	553778..554095	-	318	WP_207903173.1	addiction module toxin RelE	Toxin
E0Z06_RS02655	554401..554778	-	378	WP_132586653.1	pilin	-
E0Z06_RS02660	554853..555269	-	417	WP_132586654.1	pilin	-
E0Z06_RS02665	555660..556328	-	669	WP_132581638.1	cold shock domain-containing protein	-
E0Z06_RS02670	556442..557311	-	870	WP_132581640.1	carboxylating nicotinate-nucleotide diphosphorylase	-
E0Z06_RS02675	557314..557835	-	522	WP_132581642.1	TIGR02281 family clan AA aspartic protease	-
E0Z06_RS02680	557904..558473	+	570	WP_132581644.1	1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (44-101)

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-87)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12227.28 Da
• Isoelectric Point: 10.3340

>T120003 WP_207903173.1 NZ_CP037745:c554095-553778 [Rheinheimera sp. D18]
MFVIFVETTIFTKHLKALIPDDEYRKFQQFLVNQPEAGKVIKGSGGLRKVRWGSESQGKSGGVRVIYYYVQPNSHIRLLL
IYPKSEQTTLTDKQLKLLKDIIERW

Nucleotide

• Length: 318 bp

>T120003 NZ_CP037745:c554095-553778 [Rheinheimera sp. D18]
ATGTTTGTGATATTTGTTGAAACAACCATTTTCACCAAACACCTTAAAGCCTTGATACCTGACGATGAATATCGTAAATT
TCAACAATTTTTGGTAAATCAGCCAGAAGCTGGAAAAGTAATAAAAGGCAGCGGAGGTTTACGTAAAGTTCGCTGGGGCT
CTGAAAGTCAGGGCAAAAGCGGTGGAGTCCGAGTAATTTACTATTACGTACAGCCCAACAGTCATATACGACTATTGTTA
ATATACCCAAAGAGCGAACAAACTACTTTAACCGATAAACAGCTGAAATTATTAAAAGATATAATCGAGAGGTGGTAG

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10867.43 Da
• Isoelectric Point: 6.9835

>AT120003 WP_132581636.1 NZ_CP037745:c553776-553489 [Rheinheimera sp. D18]
MNDNLFNELLESVQQMDEIVRGERQPCRETFIAAIKVKEIRQRTNLSQAAFADLIDVQLGTLRNWEQGRREPTGPAKALL
RAIERDPKHVLAALN

Nucleotide

• Length: 288 bp

>AT120003 NZ_CP037745:c553776-553489 [Rheinheimera sp. D18]
ATGAACGACAACTTGTTTAATGAGCTTCTAGAAAGTGTGCAGCAAATGGATGAAATTGTTCGTGGTGAACGCCAGCCTTG
TCGTGAAACCTTTATTGCCGCTATTAAAGTTAAAGAAATACGTCAGCGTACGAATCTAAGTCAAGCTGCTTTTGCTGACC
TAATTGATGTTCAGTTGGGTACTTTACGTAATTGGGAACAAGGCCGTAGAGAACCAACCGGCCCAGCAAAAGCATTGTTG
CGAGCCATTGAGCGTGACCCTAAACATGTACTTGCAGCACTCAATTAA