Detailed information of TA system TA119998

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 57406..58133
• Replicon: plasmid pDHQP1701672_hv
• Accession: NZ_CP037743
• Organism: Klebsiella pneumoniae strain ST23

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7W3D9W1
• Locus tag: E1A84_RS26500
• Protein ID: WP_011251285.1
• Coordinates: 57822..58133 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: E1A84_RS26495
• Protein ID: WP_011251286.1
• Coordinates: 57406..57825 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E1A84_RS26460	52574..53044	-	471	WP_048333570.1	hypothetical protein	-
E1A84_RS29340	53237..53992	-	756	Protein_48	hypothetical protein	-
E1A84_RS26470	54588..55031	+	444	WP_048333569.1	hypothetical protein	-
E1A84_RS26475	55052..55840	+	789	WP_040217257.1	hypothetical protein	-
E1A84_RS26480	55854..56189	+	336	Protein_51	conjugal transfer protein TraI	-
E1A84_RS26485	56291..57259	-	969	WP_169050781.1	IS5 family transposase	-
E1A84_RS26495	57406..57825	-	420	WP_011251286.1	helix-turn-helix domain-containing protein	Antitoxin
E1A84_RS26500	57822..58133	-	312	WP_011251285.1	type II toxin-antitoxin system HigB family toxin	Toxin
E1A84_RS26505	58338..58796	-	459	Protein_55	DDE-type integrase/transposase/recombinase	-
E1A84_RS26510	58910..59607	+	698	Protein_56	IS1 family transposase	-
E1A84_RS26515	59624..60058	+	435	Protein_57	thioredoxin fold domain-containing protein	-
E1A84_RS26520	60075..60386	+	312	WP_011251282.1	hypothetical protein	-
E1A84_RS26525	60400..60741	+	342	WP_011251281.1	hypothetical protein	-
E1A84_RS26530	60801..61757	+	957	WP_011251280.1	DsbA family protein	-
E1A84_RS26535	61874..62449	+	576	WP_074424927.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	iroB / iroC / iroD / iroN / rmpA / iucA / iucB / iucC / iucD / iutA / rmpA2	1..227239	227239
	-	inside	IScluster/Tn	-	-	47285..68303	21018

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12346.12 Da
• Isoelectric Point: 9.7248

>T119998 WP_011251285.1 NZ_CP037743:c58133-57822 [Klebsiella pneumoniae]
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T119998 NZ_CP037743:c58133-57822 [Klebsiella pneumoniae]
ATGCACGTAATTTCAAAAGAGCCTTTTGAGGAAGCGGCAAAGCGATATCCCAACGATTCGTTAGCCATTCGGGCGTTGTA
CCGCTTAGTGCGCGAGACGGACTTCTCTTCTCCAGCTGAGATGCTAACTCTGATACCGAGCCTGGATAATTTTAAGTACA
GGAATAAGTGGTGGGTTTTGGATGTAGGGGGCAACAACTTAAGGGTTATCGCCTATATCAATTTCGTAAATAAGCGCTTC
TACGTGAAGCACATCGCAACCCACGCTGATTACGACAAGTTGACCCGCTATTACAGGGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15377.46 Da
• Isoelectric Point: 4.4420

>AT119998 WP_011251286.1 NZ_CP037743:c57825-57406 [Klebsiella pneumoniae]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT119998 NZ_CP037743:c57825-57406 [Klebsiella pneumoniae]
ATGATTACCGACACTGCAAAAGCCATTGAAGCAACCAAACAACTCGTAGCTGCCGTCCCCTTCCTAGGGGGCAGTTCTTC
CGAGAGCGATTACCGCGAAGCGATGGAGCTGGTGGATTATCTGATCGAGAACGACGACGAGAACCCACTTATTGACTTCC
TGGCGAGTAAGATTGCCGACTATGAAGATAACAGCCCTCGTTTCGCTGAGTTCAACAAAGCTATTGCTGAAATACCAGTC
GGCGTTGCACTTCTGCGAACCCTGATTGACCAGCACAAGCTGTCTTATTCAGACCTTAAGGATGAGATCGGATCTAAATC
ATTGGTCAGCCAAATCCTGTCAGGGCAGCGCTCTTTGACCATCACTCACATAAAAGCTCTTTCCGCGCGCTTCGGTGTAA
AACCTGAGTGGTTCCTATAA

Structures

Toxin

• AlphaFold DB: A0A7W3D9W1