Detailed information of TA system TA119960

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 2217648..2218250
• Replicon: chromosome
• Accession: NZ_CP037449
• Organism: Escherichia coli strain ATCC 25922

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: E0708_RS10380
• Protein ID: WP_000897302.1
• Coordinates: 2217648..2217959 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: E0708_RS10385
• Protein ID: WP_000356397.1
• Coordinates: 2217960..2218250 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E0708_RS10355	2213561..2214160	+	600	WP_001443191.1	glucose-1-phosphatase	-
E0708_RS10360	2214154..2215026	+	873	WP_000920762.1	virulence factor BrkB family protein	-
E0708_RS10365	2215023..2215460	+	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
E0708_RS10370	2215505..2216446	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
E0708_RS10375	2216510..2217418	-	909	WP_001305059.1	alpha/beta hydrolase	-
E0708_RS10380	2217648..2217959	+	312	WP_000897302.1	hypothetical protein	Toxin
E0708_RS10385	2217960..2218250	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
E0708_RS10390	2218609..2218887	+	279	WP_001296612.1	hypothetical protein	-
E0708_RS10395	2219283..2219501	+	219	WP_001314326.1	ribbon-helix-helix domain-containing protein	-
E0708_RS10400	2219717..2220646	-	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
E0708_RS10405	2220643..2221278	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
E0708_RS10410	2221275..2222177	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T119960 WP_000897302.1 NZ_CP037449:2217648-2217959 [Escherichia coli]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T119960 NZ_CP037449:2217648-2217959 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGAACGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTCTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT119960 WP_000356397.1 NZ_CP037449:2217960-2218250 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT119960 NZ_CP037449:2217960-2218250 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: S1P416