Detailed information of TA system TA119958

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 1379208..1380007
• Replicon: chromosome
• Accession: NZ_CP037449
• Organism: Escherichia coli strain ATCC 25922

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: Q8FDB4
• Locus tag: E0708_RS06330
• Protein ID: WP_000347252.1
• Coordinates: 1379543..1380007 (+)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: S1PPV5
• Locus tag: E0708_RS06325
• Protein ID: WP_001296435.1
• Coordinates: 1379208..1379543 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E0708_RS06310	1374993..1375763	-	771	WP_001058223.1	2-dehydro-3-deoxyglucarate aldolase	-
E0708_RS06315	1375779..1377113	-	1335	WP_000979025.1	galactarate/glucarate/glycerate transporter GarP	-
E0708_RS06320	1377488..1379059	+	1572	WP_001273940.1	galactarate dehydratase	-
E0708_RS06325	1379208..1379543	+	336	WP_001296435.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
E0708_RS06330	1379543..1380007	+	465	WP_000347252.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
E0708_RS06335	1380062..1380871	-	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
E0708_RS06340	1381120..1382400	+	1281	WP_000681926.1	tagatose-bisphosphate aldolase subunit KbaZ	-
E0708_RS06345	1382423..1382896	+	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
E0708_RS06350	1382907..1383686	+	780	WP_000406214.1	PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC	-
E0708_RS06355	1383676..1384554	+	879	WP_001298314.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
E0708_RS06360	1384572..1385006	+	435	WP_000948824.1	PTS sugar transporter subunit IIA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17750.11 Da
• Isoelectric Point: 9.4947

>T119958 WP_000347252.1 NZ_CP037449:1379543-1380007 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEESH

Nucleotide

• Length: 465 bp

>T119958 NZ_CP037449:1379543-1380007 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGATGCTTTAGTTGC
CGAAGTCGAGGCATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCTGCCACAAAGTTATTGGCGGTGGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGCGCTGGTCGCTATCGTCTCTTCTTTCGTTACAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGTAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAGACAGAAGAAAGCCATTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12321.92 Da
• Isoelectric Point: 4.5392

>AT119958 WP_001296435.1 NZ_CP037449:1379208-1379543 [Escherichia coli]
MPANARSNAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFDIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT119958 NZ_CP037449:1379208-1379543 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTAACGCTGTACTGACCACTGAATCAAAGGTCACTATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCTGGCCTGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCGACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGACGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: Q8FDB4

Antitoxin

• AlphaFold DB: S1PPV5