Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 81604..82106 Replicon chromosome
Accession NZ_CP037449
Organism Escherichia coli strain ATCC 25922

Toxin (Protein)


Gene name yoeB Uniprot ID S1P977
Locus tag E0708_RS00375 Protein ID WP_000767822.1
Coordinates 81604..81858 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag E0708_RS00380 Protein ID WP_001259255.1
Coordinates 81855..82106 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E0708_RS00355 78097..79455 - 1359 WP_000019194.1 putrescine/proton symporter PlaP -
E0708_RS00360 79445..79507 - 63 WP_010723108.1 membrane protein YoeI -
E0708_RS00365 79722..80651 - 930 WP_000803366.1 LysR family transcriptional regulator -
E0708_RS00370 80697..81521 - 825 WP_000754733.1 SDR family oxidoreductase -
E0708_RS00375 81604..81858 - 255 WP_000767822.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
E0708_RS00380 81855..82106 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
E0708_RS00385 82389..82439 + 51 WP_001364200.1 his operon leader peptide -
E0708_RS00390 82585..83484 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
E0708_RS00395 83490..84794 + 1305 WP_001297912.1 histidinol dehydrogenase -
E0708_RS00400 84791..85861 + 1071 WP_000108995.1 histidinol-phosphate transaminase -
E0708_RS00405 85861..86928 + 1068 WP_000080122.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(5-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T119954 WP_000767822.1 NZ_CP037449:c81858-81604 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINEIIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T119954 NZ_CP037449:c81858-81604 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAAATTATCAAAGATACACGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT119954 WP_001259255.1 NZ_CP037449:c82106-81855 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT119954 NZ_CP037449:c82106-81855 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1P977

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References