Detailed information of TA system TA119897

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-MqsA
• Location: 327381..328014
• Replicon: chromosome
• Accession: NZ_CP036500
• Organism: Vibrio cholerae strain 20000

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q9KMA6
• Locus tag: EZZ25_RS15325
• Protein ID: WP_000843587.1
• Coordinates: 327381..327713 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: EZZ25_RS15330
• Protein ID: WP_000071010.1
• Coordinates: 327700..328014 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EZZ25_RS15290	322551..322904	+	354	WP_001138366.1	50S ribosomal protein L20	-
EZZ25_RS15295	322986..323947	-	962	Protein_280	integron integrase IntIA	-
EZZ25_RS15300	324230..324466	-	237	WP_000772130.1	RNA-binding protein	-
EZZ25_RS15305	324720..325220	+	501	WP_000686368.1	NAD(P)H-dependent oxidoreductase	-
EZZ25_RS15310	325357..326304	+	948	WP_000433728.1	hypothetical protein	-
EZZ25_RS15315	326462..327205	+	744	WP_000188760.1	toll/interleukin-1 receptor domain-containing protein	-
EZZ25_RS15325	327381..327713	+	333	WP_000843587.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EZZ25_RS15330	327700..328014	+	315	WP_000071010.1	DNA-binding transcriptional regulator	Antitoxin
EZZ25_RS15335	328134..328796	+	663	WP_000084912.1	Vat family streptogramin A O-acetyltransferase	-
EZZ25_RS15340	328934..329416	+	483	WP_001090702.1	hypothetical protein	-
EZZ25_RS15345	329596..329871	+	276	WP_032473764.1	hypothetical protein	-
EZZ25_RS19415	330222..330317	+	96	WP_001905766.1	DUF645 family protein	-
EZZ25_RS15365	330497..331012	+	516	WP_000090017.1	hypothetical protein	-
EZZ25_RS15370	331306..331487	+	182	Protein_292	DUF645 family protein	-
EZZ25_RS15375	331686..332432	+	747	WP_000846952.1	hypothetical protein	-
EZZ25_RS15380	332578..333012	+	435	WP_000256035.1	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integron	-	-	322986..403614	80628

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (4-100)

Antitoxin

• Antitoxin MqsA/HigA-2 (28-101)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 13005.95 Da
• Isoelectric Point: 9.9244

>T119897 WP_000843587.1 NZ_CP036500:327381-327713 [Vibrio cholerae]
MKSVFVESTIFEKYRDEYLSDEEYRLFQAELMLNPKLGDVIQGTGGLRKIRVASKGKGKRGGSRIIYYFLDEKRRFYLLT
IYGKNEMSDLNANQRKQLMAFMEAWRNEQS

Nucleotide

• Length: 333 bp

>T119897 NZ_CP036500:327381-327713 [Vibrio cholerae]
ATGAAAAGTGTATTTGTCGAATCAACAATTTTTGAAAAGTACCGAGATGAATATCTCAGTGATGAGGAGTATAGGCTCTT
TCAAGCAGAGCTAATGCTAAACCCCAAGCTGGGTGATGTGATTCAAGGTACTGGCGGTTTGCGAAAAATTCGAGTTGCGA
GTAAAGGCAAGGGAAAGCGTGGTGGTTCACGGATTATCTATTACTTTCTCGATGAAAAGAGGCGTTTCTATTTGCTAACC
ATTTACGGCAAAAATGAAATGTCTGACTTGAATGCAAATCAAAGGAAACAACTAATGGCTTTTATGGAGGCGTGGCGCAA
TGAGCAATCGTGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11687.19 Da
• Isoelectric Point: 6.6445

>AT119897 WP_000071010.1 NZ_CP036500:327700-328014 [Vibrio cholerae]
MSNRDLFAELSSALVEAKQHSEGKLTLKTHHVNDVGELNISPDEIVSIREQFNMSRGVFARLLHTSSRTLENWEQGRSVP
NGQAVTLLKLVQRHPETLSQIAEL

Nucleotide

• Length: 315 bp

>AT119897 NZ_CP036500:327700-328014 [Vibrio cholerae]
ATGAGCAATCGTGATTTATTTGCAGAGTTAAGCTCAGCTCTTGTTGAGGCTAAGCAGCATTCAGAAGGTAAGCTTACTCT
GAAAACACATCATGTGAATGATGTGGGTGAGTTGAACATCTCACCGGATGAAATCGTGAGTATTCGCGAGCAGTTCAATA
TGTCCCGCGGAGTCTTCGCGCGGTTACTTCATACGTCTTCGCGCACATTAGAAAACTGGGAACAAGGTCGTAGCGTGCCA
AATGGTCAAGCGGTCACTCTTTTAAAGTTAGTACAGCGTCATCCAGAAACGTTGTCACAAATAGCCGAGCTATAA

Structures

Toxin

• PDB: 5JA9
• PDB: 8A0X
• PDB: 5MJE
• PDB: 5JA8
• PDB: 5JAA