Detailed information of TA system TA119888

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2006517..2007130
• Replicon: chromosome
• Accession: NZ_CP036498
• Organism: Rhodopseudomonas sp. SK50-23

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: RPMA_RS09440
• Protein ID: WP_211912564.1
• Coordinates: 2006517..2006813 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: RPMA_RS09445
• Protein ID: WP_211912565.1
• Coordinates: 2006822..2007130 (+)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RPMA_RS09420 (RPMA_09370)	2002522..2002722	+	201	WP_211912560.1	DUF2065 domain-containing protein	-
RPMA_RS09425 (RPMA_09375)	2002910..2004424	+	1515	WP_211912561.1	Do family serine endopeptidase	-
RPMA_RS09430 (RPMA_09380)	2004635..2005537	-	903	WP_211912562.1	phosphoserine phosphatase SerB	-
RPMA_RS09435 (RPMA_09385)	2005541..2006464	+	924	WP_211912563.1	tRNA (adenosine(37)-N6)-dimethylallyltransferase MiaA	-
RPMA_RS09440 (RPMA_09390)	2006517..2006813	+	297	WP_211912564.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
RPMA_RS09445 (RPMA_09395)	2006822..2007130	+	309	WP_211912565.1	HigA family addiction module antitoxin	Antitoxin
RPMA_RS09450 (RPMA_09400)	2007198..2008298	-	1101	WP_211912566.1	tyrosinase family protein	-
RPMA_RS09455 (RPMA_09405)	2008307..2008684	+	378	WP_211912567.1	hypothetical protein	-
RPMA_RS09460 (RPMA_09410)	2008870..2010648	+	1779	WP_211912568.1	acetolactate synthase 3 large subunit	-
RPMA_RS09465 (RPMA_09415)	2010844..2011386	+	543	WP_211912569.1	acetolactate synthase small subunit	-
RPMA_RS09470 (RPMA_09420)	2011442..2011804	+	363	WP_211912570.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-97)

Antitoxin

• Cro/C1-type helix-turn-helix domain (18-69)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11591.09 Da
• Isoelectric Point: 6.7252

>T119888 WP_211912564.1 NZ_CP036498:2006517-2006813 [Rhodopseudomonas sp. SK50-23]
MIVGFRDGWLQSFFVDDVRSRHIPSDLESRLFRRLQMIDDAVTDLDLRMPPSNHFEKLRGTLAGFYSIRVNDQWRLIFRW
DGARGEAAGVYLDDHSYR

Nucleotide

• Length: 297 bp

>T119888 NZ_CP036498:2006517-2006813 [Rhodopseudomonas sp. SK50-23]
ATGATCGTAGGATTTCGCGATGGCTGGCTGCAATCCTTCTTCGTGGACGATGTACGTTCTCGCCATATTCCGAGCGACCT
CGAAAGTCGGCTGTTTCGTAGACTTCAGATGATCGATGACGCTGTCACCGACCTGGATCTCCGCATGCCGCCGAGCAATC
ATTTCGAGAAGCTGCGGGGCACGCTTGCTGGCTTTTACTCGATCCGTGTCAACGATCAGTGGCGGCTGATTTTCCGCTGG
GACGGCGCCCGAGGTGAGGCCGCTGGTGTTTATCTCGACGATCATAGTTATAGGTGA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11454.30 Da
• Isoelectric Point: 10.5835

>AT119888 WP_211912565.1 NZ_CP036498:2006822-2007130 [Rhodopseudomonas sp. SK50-23]
MLMTKRKPATIGEILSEEFMQPLGLTQGALADAMGVQRKHVNELCNDRRGVTAATALILARVFGNSPDFWLNVQRRNDLW
QAMHSPEERARIERAKPLVKAA

Nucleotide

• Length: 309 bp

>AT119888 NZ_CP036498:2006822-2007130 [Rhodopseudomonas sp. SK50-23]
ATGCTCATGACCAAGCGCAAGCCGGCGACGATTGGCGAGATTCTCTCTGAGGAATTCATGCAGCCGCTCGGCCTGACGCA
GGGCGCGCTTGCTGATGCCATGGGCGTACAGCGCAAGCATGTGAACGAGCTATGCAATGATCGCCGCGGCGTGACAGCCG
CAACCGCGCTCATTCTCGCGCGCGTATTTGGCAATAGTCCGGACTTCTGGCTGAACGTTCAGCGCCGAAATGATCTCTGG
CAAGCGATGCATTCCCCCGAAGAACGTGCCCGGATCGAACGCGCCAAACCGCTGGTGAAGGCGGCGTGA