Detailed information of TA system TA119884

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4467375..4467976
• Replicon: chromosome
• Accession: NZ_CP036495
• Organism: Pseudomonas viridiflava strain Psy35

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A5E6ZPL8
• Locus tag: EZZ81_RS19890
• Protein ID: WP_029241687.1
• Coordinates: 4467375..4467689 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: EZZ81_RS19895
• Protein ID: WP_029241688.1
• Coordinates: 4467689..4467976 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EZZ81_RS19880 (EZZ81_20155)	4463798..4465129	+	1332	WP_029241685.1	AcvB/VirJ family lysyl-phosphatidylglycerol hydrolase	-
EZZ81_RS19885 (EZZ81_20160)	4465211..4467106	-	1896	WP_029241686.1	potassium transporter Kup	-
EZZ81_RS19890 (EZZ81_20165)	4467375..4467689	+	315	WP_029241687.1	hypothetical protein	Toxin
EZZ81_RS19895 (EZZ81_20170)	4467689..4467976	+	288	WP_029241688.1	helix-turn-helix domain-containing protein	Antitoxin
EZZ81_RS19900 (EZZ81_20175)	4468132..4469475	+	1344	WP_004879110.1	30S ribosomal protein S12 methylthiotransferase RimO	-
EZZ81_RS19905 (EZZ81_20180)	4469580..4470023	+	444	WP_029241689.1	GNAT family N-acetyltransferase	-
EZZ81_RS19910 (EZZ81_20185)	4470115..4470825	+	711	WP_029241690.1	rRNA pseudouridine synthase	-
EZZ81_RS19915 (EZZ81_20190)	4470895..4471356	+	462	WP_029241691.1	DUF1456 family protein	-
EZZ81_RS19920 (EZZ81_20195)	4471366..4472073	+	708	WP_029241692.1	tRNA (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase TrmO	-
EZZ81_RS19925 (EZZ81_20200)	4472122..4472901	-	780	WP_002554680.1	ferredoxin-NADP reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-88)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11897.75 Da
• Isoelectric Point: 10.7948

>T119884 WP_029241687.1 NZ_CP036495:4467375-4467689 [Pseudomonas viridiflava]
MIFIETPIFTSELRTHLSDDEYRALQSHLASQPEAGALIEDTGGLRKVRWTAHGKGKSGGVRVIYYHLSSANQIRMLLIY
RKGIMDSLTAKQKTQLRAINRRWK

Nucleotide

• Length: 315 bp

>T119884 NZ_CP036495:4467375-4467689 [Pseudomonas viridiflava]
ATGATTTTTATCGAAACACCTATATTCACTTCAGAGCTCAGGACTCATCTGAGCGACGACGAATATCGCGCGCTTCAATC
GCACCTCGCATCCCAGCCGGAAGCTGGCGCGCTGATTGAAGACACAGGAGGATTGAGGAAGGTACGCTGGACTGCGCATG
GGAAAGGCAAGAGTGGTGGGGTAAGGGTGATTTATTACCACTTATCCTCTGCCAATCAGATACGAATGCTTTTGATCTAT
CGAAAAGGGATCATGGATTCCCTGACTGCGAAGCAAAAGACACAGCTGCGAGCAATCAACAGAAGGTGGAAATAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10919.38 Da
• Isoelectric Point: 7.3222

>AT119884 WP_029241688.1 NZ_CP036495:4467689-4467976 [Pseudomonas viridiflava]
MEKNLFDRLVESMTQMDEIDRDERQPSREFHVDAVQVKKIRQATGLSQASFAKRIDVAVGTLRNWEQGRREPEGPARALL
KAIHNDPEHVLAALR

Nucleotide

• Length: 288 bp

>AT119884 NZ_CP036495:4467689-4467976 [Pseudomonas viridiflava]
ATGGAAAAGAATCTTTTTGATCGTCTTGTGGAAAGCATGACGCAAATGGACGAAATTGATCGCGATGAGCGCCAGCCTTC
CCGCGAGTTCCACGTTGATGCAGTTCAGGTAAAGAAGATCCGCCAGGCTACAGGGCTATCCCAGGCGAGCTTCGCCAAAC
GCATAGACGTGGCGGTAGGTACGCTTAGGAACTGGGAGCAAGGTCGCCGCGAACCTGAGGGGCCTGCCAGAGCCTTGTTA
AAGGCCATACACAACGACCCGGAACACGTATTGGCGGCGCTTCGCTGA

Structures

Toxin

• AlphaFold DB: A0A5E6ZPL8