Detailed information of TA system TA119881

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemK-chpS/PRK09812-ChpS
• Location: 446654..447231
• Replicon: chromosome
• Accession: NZ_CP036495
• Organism: Pseudomonas viridiflava strain Psy35

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: EZZ81_RS02140
• Protein ID: WP_080665327.1
• Coordinates: 446905..447231 (+)
• Length: 109 a.a.

Antitoxin (Protein)

• Gene name: chpS
• Uniprot ID: A0A5E7C1C9
• Locus tag: EZZ81_RS02135
• Protein ID: WP_004880245.1
• Coordinates: 446654..446908 (+)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EZZ81_RS02115 (EZZ81_02160)	441916..443307	+	1392	WP_029244144.1	heavy metal sensor histidine kinase	-
EZZ81_RS02120 (EZZ81_02165)	443404..443805	+	402	WP_004880251.1	MAPEG family protein	-
EZZ81_RS02125 (EZZ81_02175)	444266..444673	-	408	WP_025995654.1	DUF1090 domain-containing protein	-
EZZ81_RS02130 (EZZ81_02180)	444728..446410	-	1683	WP_029244143.1	NAD-dependent DNA ligase LigB	-
EZZ81_RS02135 (EZZ81_02185)	446654..446908	+	255	WP_004880245.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
EZZ81_RS02140 (EZZ81_02190)	446905..447231	+	327	WP_080665327.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
EZZ81_RS02145 (EZZ81_02195)	447176..448366	-	1191	WP_004880242.1	methionine adenosyltransferase	-
EZZ81_RS02150 (EZZ81_02200)	448387..449382	-	996	WP_029244142.1	metalloregulator ArsR/SmtB family transcription factor	-
EZZ81_RS02155 (EZZ81_02205)	449641..451638	+	1998	WP_029244141.1	transketolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-73)

Antitoxin

• SpoVT-AbrB domain (6-48)

Sequences

Toxin

Protein

• Length: 109 a.a.
• Molecular weight: 11913.60 Da
• Isoelectric Point: 12.3548

>T119881 WP_080665327.1 NZ_CP036495:446905-447231 [Pseudomonas viridiflava]
VKQHGFNRADIVRISLNPTAGREQQRDFRPALVLTPAAYNVTGLAIVAPIIQGGDFARYSGFAVPLGGAGVPNKSPRRLT
SPGAFDDRRRITDRQQRAGRRRGRFFPK

Nucleotide

• Length: 327 bp

>T119881 NZ_CP036495:446905-447231 [Pseudomonas viridiflava]
GTGAAGCAGCACGGATTCAATCGCGCAGATATCGTCCGCATCAGCCTCAATCCAACCGCTGGCCGAGAACAGCAGCGCGA
TTTTCGTCCCGCGTTGGTGCTGACACCAGCCGCGTATAACGTCACCGGCCTTGCGATCGTTGCGCCTATCATACAGGGCG
GTGATTTTGCGCGTTATTCGGGGTTCGCTGTTCCTTTGGGTGGGGCGGGAGTCCCAAACAAAAGCCCCCGGAGACTCACG
TCGCCGGGGGCTTTTGATGACCGCAGGAGAATTACAGACCGGCAGCAGCGCGCAGGGCGTCGGCGCGGTCGGTTTTTTCC
CAAGTGA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9217.72 Da
• Isoelectric Point: 4.6471

>AT119881 WP_004880245.1 NZ_CP036495:446654-446908 [Pseudomonas viridiflava]
MEVKIQQWGNSAAIRLPANVLKQMSLVIGDVLVLDVSSEVMTLKPAKARLHYQLADLMAQCDLNASEPEELAGWNAMQPI
GREA

Nucleotide

• Length: 255 bp

>AT119881 NZ_CP036495:446654-446908 [Pseudomonas viridiflava]
ATGGAAGTCAAAATTCAGCAGTGGGGCAATAGCGCAGCAATCAGATTGCCAGCCAATGTGCTCAAGCAGATGAGCCTGGT
CATCGGTGATGTGCTGGTGCTGGATGTTTCCAGCGAAGTGATGACGCTCAAGCCTGCCAAGGCCAGACTGCATTACCAAT
TGGCCGACCTTATGGCCCAATGTGATTTGAATGCGTCCGAGCCAGAGGAGTTGGCTGGTTGGAACGCCATGCAACCCATA
GGGCGTGAAGCGTGA

Structures

Antitoxin

• AlphaFold DB: A0A5E7C1C9