Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HTH(antitoxin)
Location 4822798..4823399 Replicon chromosome
Accession NZ_CP036494
Organism Pseudomonas putida strain PP1

Toxin (Protein)


Gene name higB Uniprot ID A0A2L1IAQ2
Locus tag EZZ80_RS22295 Protein ID WP_049587984.1
Coordinates 4822798..4823112 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A2L1IAR7
Locus tag EZZ80_RS22300 Protein ID WP_049587986.1
Coordinates 4823112..4823399 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EZZ80_RS22285 (EZZ80_22745) 4819098..4820390 + 1293 WP_010952354.1 AcvB/VirJ family lysyl-phosphatidylglycerol hydrolase -
EZZ80_RS22290 (EZZ80_22750) 4820439..4822349 - 1911 WP_010952353.1 potassium transporter Kup -
EZZ80_RS22295 (EZZ80_22755) 4822798..4823112 + 315 WP_049587984.1 hypothetical protein Toxin
EZZ80_RS22300 (EZZ80_22760) 4823112..4823399 + 288 WP_049587986.1 helix-turn-helix domain-containing protein Antitoxin
EZZ80_RS22305 (EZZ80_22765) 4823600..4824931 + 1332 WP_004576169.1 30S ribosomal protein S12 methylthiotransferase RimO -
EZZ80_RS22310 (EZZ80_22770) 4825003..4825752 - 750 WP_049587988.1 hypothetical protein -
EZZ80_RS22315 (EZZ80_22775) 4825807..4827525 - 1719 WP_010952348.1 DUF5666 domain-containing protein -
EZZ80_RS22320 (EZZ80_22780) 4827522..4828367 - 846 WP_003254799.1 DUF6502 family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Cro/C1-type helix-turn-helix domain

(36-88)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11784.61 Da        Isoelectric Point: 9.9097

>T119879 WP_049587984.1 NZ_CP036494:4822798-4823112 [Pseudomonas putida]
MIFIETPVFTSDLKEHLDDEEYRALQAYLAEHPEAGSLLEETGGLRKIRWAAKGKGKSGGVRVIYYHVTAAHQIRMILIY
RKGIVDTLTSSQKAQLRALNKGWK

Download         Length: 315 bp

>T119879 NZ_CP036494:4822798-4823112 [Pseudomonas putida]
ATGATCTTCATCGAGACCCCAGTATTCACCTCCGATCTCAAGGAGCACCTGGATGATGAAGAATACCGGGCTTTGCAGGC
CTATCTGGCCGAGCATCCCGAAGCCGGTTCTCTCCTGGAGGAAACCGGCGGCCTCAGAAAGATCCGCTGGGCAGCCAAGG
GCAAGGGCAAGAGTGGGGGTGTACGAGTGATCTACTACCACGTCACTGCAGCCCACCAGATCCGTATGATTCTGATCTAT
CGCAAGGGCATCGTGGATACCTTGACAAGCAGTCAGAAAGCGCAGCTTCGCGCACTAAACAAAGGGTGGAAATAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10841.31 Da        Isoelectric Point: 7.4063

>AT119879 WP_049587986.1 NZ_CP036494:4823112-4823399 [Pseudomonas putida]
MEKHLFDRLVESMTQMDEIDRGERQPSREFHVDALQVKKIRQATGLSQAAFAKRIDVAVGTLRNWEQGRREPEGPARALL
RAIHNDPEHVLAALS

Download         Length: 288 bp

>AT119879 NZ_CP036494:4823112-4823399 [Pseudomonas putida]
ATGGAAAAGCATCTCTTCGATCGTCTTGTGGAAAGCATGACTCAGATGGATGAGATCGATCGCGGTGAGCGTCAGCCCTC
GCGTGAGTTCCACGTAGACGCACTTCAAGTCAAAAAGATTCGTCAAGCCACTGGCCTTTCCCAGGCTGCGTTTGCCAAAC
GTATCGATGTCGCAGTAGGAACTCTACGGAATTGGGAACAGGGCCGTCGCGAACCTGAAGGACCAGCTCGTGCCTTGCTT
CGAGCGATTCATAACGATCCCGAACATGTACTTGCGGCCTTGAGCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2L1IAQ2

Antitoxin

Source ID Structure
AlphaFold DB A0A2L1IAR7

References