Detailed information of TA system TA119878

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 4746725..4747515
• Replicon: chromosome
• Accession: NZ_CP036494
• Organism: Pseudomonas putida strain PP1

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: Q88NE5
• Locus tag: EZZ80_RS21935
• Protein ID: WP_010952397.1
• Coordinates: 4747048..4747515 (+)
• Length: 156 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: I7C311
• Locus tag: EZZ80_RS21930
• Protein ID: WP_014859917.1
• Coordinates: 4746725..4747051 (+)
• Length: 109 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EZZ80_RS21910 (EZZ80_22360)	4742259..4743320	-	1062	WP_049587435.1	HlyD family secretion protein	-
EZZ80_RS21915 (EZZ80_22365)	4743349..4744890	-	1542	WP_010952401.1	MFS transporter	-
EZZ80_RS21920 (EZZ80_22370)	4745007..4745912	-	906	WP_010952400.1	LysR family transcriptional regulator	-
EZZ80_RS21925 (EZZ80_22380)	4746186..4746632	+	447	WP_010952399.1	universal stress protein	-
EZZ80_RS21930 (EZZ80_22385)	4746725..4747051	+	327	WP_014859917.1	type II toxin-antitoxin system PrlF family antitoxin	Antitoxin
EZZ80_RS21935 (EZZ80_22390)	4747048..4747515	+	468	WP_010952397.1	type II toxin-antitoxin system YhaV family toxin	Toxin
EZZ80_RS21940 (EZZ80_22395)	4747588..4748448	-	861	WP_010952396.1	HlyD family secretion protein	-
EZZ80_RS21945 (EZZ80_22400)	4748459..4748659	-	201	WP_003251718.1	DUF1656 domain-containing protein	-
EZZ80_RS21950 (EZZ80_22405)	4748649..4750826	-	2178	WP_003251716.1	FUSC family protein	-
EZZ80_RS21955 (EZZ80_22410)	4750823..4752352	-	1530	WP_010952395.1	efflux transporter outer membrane subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (17-152)

Antitoxin

• PrlF antitoxin (8-106)

Sequences

Toxin

Protein

• Length: 156 a.a.
• Molecular weight: 17962.34 Da
• Isoelectric Point: 9.7963

>T119878 WP_010952397.1 NZ_CP036494:4747048-4747515 [Pseudomonas putida]
MSDASRKPLVIHGWTVIAHPLFLAKLDALSAQVEAQRDKDPTGYTKRNTFKRLAAIRRLAFDVIPQDPTKPEYRQGATLG
GDHKHWFRAKFFQQYRLFFRYHTPSRIIVLAWINDESSKRAYESSDDAYKVFQKMLHSGNPPDDWDQLLQEAAAD

Nucleotide

• Length: 468 bp

>T119878 NZ_CP036494:4747048-4747515 [Pseudomonas putida]
ATGAGTGATGCGAGCAGGAAGCCTCTGGTTATTCATGGATGGACTGTAATTGCCCACCCGCTGTTCCTGGCCAAACTGGA
CGCGCTAAGCGCTCAAGTCGAGGCTCAACGGGACAAAGACCCAACGGGTTATACGAAAAGAAATACCTTTAAAAGGCTGG
CCGCCATCCGGCGGTTGGCGTTCGATGTAATCCCTCAAGACCCGACCAAACCCGAATACCGGCAAGGGGCAACACTTGGC
GGTGACCACAAGCACTGGTTCAGAGCAAAGTTCTTCCAGCAGTACCGGTTGTTCTTCCGCTACCACACCCCCAGCAGGAT
CATCGTGCTGGCCTGGATCAATGACGAGTCGAGCAAGCGGGCCTATGAAAGTAGCGACGATGCTTACAAGGTCTTTCAAA
AGATGTTGCATAGCGGCAACCCACCGGACGATTGGGACCAGTTGCTGCAAGAGGCTGCCGCAGATTGA

Antitoxin

Protein

• Length: 109 a.a.
• Molecular weight: 11919.40 Da
• Isoelectric Point: 4.3722

>AT119878 WP_014859917.1 NZ_CP036494:4746725-4747051 [Pseudomonas putida]
MAATFDVESTLTDRYQTTVPETVRRALGLKKRDKIHYSIRPDGGVILTRAEATEDDPVLGKFLDFLAHDIATNPQRLQVV
DAGLIARLDKLVGDAEVDLDQPLSADDE

Nucleotide

• Length: 327 bp

>AT119878 NZ_CP036494:4746725-4747051 [Pseudomonas putida]
ATGGCTGCCACCTTCGACGTCGAGTCCACGCTGACCGATCGCTATCAAACCACTGTTCCCGAAACGGTGCGGCGCGCCTT
GGGCTTGAAAAAACGTGACAAGATCCATTACTCGATTCGCCCCGACGGTGGGGTGATACTCACCCGCGCAGAGGCAACCG
AGGACGATCCGGTACTGGGCAAATTTCTGGATTTCCTGGCACACGATATCGCTACCAACCCTCAACGGCTGCAGGTTGTC
GATGCTGGCCTCATCGCGCGCCTCGACAAGCTTGTCGGCGACGCGGAGGTCGACCTGGATCAACCTCTGTCGGCGGACGA
TGAATGA

Structures

Toxin

• AlphaFold DB: Q88NE5

Antitoxin

• AlphaFold DB: I7C311