Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 4418583..4419169 Replicon chromosome
Accession NZ_CP036494
Organism Pseudomonas putida strain PP1

Toxin (Protein)


Gene name graT Uniprot ID Q88MI5
Locus tag EZZ80_RS20295 Protein ID WP_010952670.1
Coordinates 4418583..4418861 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name graA Uniprot ID Q88MI6
Locus tag EZZ80_RS20300 Protein ID WP_010952669.1
Coordinates 4418870..4419169 (+) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EZZ80_RS20285 (EZZ80_20700) 4415384..4416580 + 1197 WP_010952672.1 succinyldiaminopimelate transaminase -
EZZ80_RS20290 (EZZ80_20710) 4416686..4418332 - 1647 WP_049587427.1 Na+/H+ antiporter -
EZZ80_RS20295 (EZZ80_20715) 4418583..4418861 + 279 WP_010952670.1 type II toxin-antitoxin system toxin GraT Toxin
EZZ80_RS20300 (EZZ80_20720) 4418870..4419169 + 300 WP_010952669.1 type II toxin-antitoxin system antitoxin GraA Antitoxin
EZZ80_RS20305 (EZZ80_20725) 4419461..4419763 - 303 WP_049587425.1 hypothetical protein -
EZZ80_RS20310 (EZZ80_20730) 4419829..4420329 - 501 WP_010952668.1 DUF2514 domain-containing protein -
EZZ80_RS20315 (EZZ80_20735) 4420326..4420769 - 444 WP_049587424.1 structural protein P5 -
EZZ80_RS20320 (EZZ80_20740) 4420830..4421906 - 1077 WP_010952666.1 hypothetical protein -
EZZ80_RS20325 (EZZ80_20745) 4421918..4423909 - 1992 WP_010952665.1 sialidase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(1-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(20-69)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10446.72 Da        Isoelectric Point: 7.2450

>T119877 WP_010952670.1 NZ_CP036494:4418583-4418861 [Pseudomonas putida]
MIRSFSCADTEALFTTGKTRRGSDIKSVAERKLAMLDAATELRDLRSPPGNRLESLSGNRADQHSIRVNDQWRLCFTWTE
HGPVNVEIVDYH

Download         Length: 279 bp

>T119877 NZ_CP036494:4418583-4418861 [Pseudomonas putida]
ATGATTCGAAGCTTTAGCTGTGCCGACACGGAAGCGCTCTTCACAACGGGAAAGACACGGCGCGGGTCAGATATCAAGTC
AGTCGCGGAGCGCAAGCTGGCCATGCTCGATGCCGCAACAGAGTTGCGAGACCTTAGGTCGCCACCTGGCAATCGCCTCG
AGTCACTGAGCGGCAACCGCGCCGACCAGCACAGTATCCGTGTAAATGACCAATGGCGGTTGTGCTTCACCTGGACAGAG
CACGGGCCAGTTAACGTTGAAATTGTCGATTACCACTGA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10905.55 Da        Isoelectric Point: 5.6793

>AT119877 WP_010952669.1 NZ_CP036494:4418870-4419169 [Pseudomonas putida]
MLKNGMRPIHPGEILREEFQKEMGFSAAALARALGVATPTVNNILRERGGVSADMALRLSICLDTTPEFWLNLQTAFDLR
TAEQQHGDEIIGSVQRLVA

Download         Length: 300 bp

>AT119877 NZ_CP036494:4418870-4419169 [Pseudomonas putida]
ATGCTCAAGAACGGTATGCGTCCAATCCATCCGGGTGAAATCCTGCGGGAGGAGTTCCAGAAGGAAATGGGCTTCAGCGC
CGCGGCTCTGGCCAGGGCGTTGGGTGTAGCAACGCCAACCGTCAACAACATCCTGCGCGAACGGGGCGGTGTTTCCGCCG
ACATGGCACTGCGCCTGTCCATCTGCCTGGACACCACCCCTGAGTTCTGGCTCAACCTGCAGACGGCCTTCGATCTGCGC
ACAGCCGAACAGCAACACGGCGATGAGATCATCGGGTCGGTCCAGCGTCTGGTCGCTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6F8S

Antitoxin

Source ID Structure
PDB 6F8S
AlphaFold DB Q88MI6

References