Detailed information of TA system TA119872

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mqsRA (relBE)/MqsR-MqsA
• Location: 1443707..1444407
• Replicon: chromosome
• Accession: NZ_CP036494
• Organism: Pseudomonas putida strain PP1

Toxin (Protein)

• Gene name: mqsR
• Uniprot ID: Q88F93
• Locus tag: EZZ80_RS06530
• Protein ID: WP_010954960.1
• Coordinates: 1443707..1444003 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: mqsA
• Uniprot ID: I7AUJ7
• Locus tag: EZZ80_RS06535
• Protein ID: WP_003254235.1
• Coordinates: 1444006..1444407 (+)
• Length: 134 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EZZ80_RS06510 (EZZ80_06665)	1439699..1440877	-	1179	WP_010954964.1	efflux RND transporter periplasmic adaptor subunit	-
EZZ80_RS06515 (EZZ80_06670)	1441090..1441566	+	477	WP_010954963.1	sigma-70 family RNA polymerase sigma factor	-
EZZ80_RS06520 (EZZ80_06675)	1441735..1442214	+	480	WP_010954962.1	hypothetical protein	-
EZZ80_RS06525 (EZZ80_06680)	1442287..1443612	+	1326	WP_010954961.1	MFS transporter	-
EZZ80_RS06530 (EZZ80_06685)	1443707..1444003	+	297	WP_010954960.1	type II toxin-antitoxin system MqsR family toxin	Toxin
EZZ80_RS06535 (EZZ80_06690)	1444006..1444407	+	402	WP_003254235.1	type II toxin-antitoxin system MqsA family antitoxin	Antitoxin
EZZ80_RS06540 (EZZ80_06695)	1444479..1446161	-	1683	WP_010954959.1	electron transfer flavoprotein-ubiquinone oxidoreductase	-
EZZ80_RS06545 (EZZ80_06700)	1446697..1447446	+	750	WP_003254232.1	electron transfer flavoprotein subunit beta/FixA family protein	-
EZZ80_RS06550 (EZZ80_06705)	1447447..1448376	+	930	WP_010954958.1	FAD-binding protein	-
EZZ80_RS06555 (EZZ80_06710)	1448452..1449273	+	822	WP_010954957.1	transporter substrate-binding domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin MqsR (6-97)

Antitoxin

• Cro/C1-type helix-turn-helix domain (74-109)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11133.22 Da
• Isoelectric Point: 10.2271

>T119872 WP_010954960.1 NZ_CP036494:1443707-1444003 [Pseudomonas putida]
MEKRMPHCPLERVKALAAARRIRPTGAALKGAKALGMDFPGMLEVITSLKRTDFYKSMTSHIDHRVWQDVYRPLTAIGYV
YLKLSVVDDVLIVSFKEL

Nucleotide

• Length: 297 bp

>T119872 NZ_CP036494:1443707-1444003 [Pseudomonas putida]
ATGGAAAAGAGAATGCCTCATTGCCCGCTGGAGCGGGTAAAAGCCCTGGCTGCGGCAAGGCGAATCCGCCCTACGGGTGC
TGCATTGAAAGGCGCCAAGGCGCTGGGAATGGATTTTCCGGGCATGCTTGAGGTGATCACCAGCTTGAAGCGCACTGATT
TCTACAAGAGCATGACCAGCCATATTGACCACCGGGTTTGGCAGGATGTGTATCGCCCGCTTACAGCAATTGGGTACGTT
TATTTGAAATTGTCCGTGGTAGACGACGTGCTCATCGTGTCTTTCAAGGAGTTGTAA

Antitoxin

Protein

• Length: 134 a.a.
• Molecular weight: 14684.76 Da
• Isoelectric Point: 4.9531

>AT119872 WP_003254235.1 NZ_CP036494:1444006-1444407 [Pseudomonas putida]
MRCPICGGSELAPDIQGMPYSYKGEMTVIPEVSGDYCSACGECVLSHDEAMRVSHLMTAFERQVNANVVDPSFIASIRRK
FDLDQREAGEIFGGGVNAFSRYENGKTTPPVALVKLLKLLDRHPELFEEVRTA

Nucleotide

• Length: 402 bp

>AT119872 NZ_CP036494:1444006-1444407 [Pseudomonas putida]
ATGAGATGTCCAATATGCGGCGGCTCGGAGCTTGCACCTGACATTCAGGGCATGCCCTATAGCTACAAAGGCGAGATGAC
AGTGATTCCTGAGGTAAGTGGCGATTACTGCTCCGCATGTGGTGAATGTGTACTGAGTCATGATGAAGCCATGCGCGTCA
GTCACTTGATGACGGCATTCGAGCGCCAGGTCAACGCAAATGTTGTGGATCCTTCCTTTATTGCCTCCATACGCAGAAAG
TTCGACCTCGACCAGCGTGAAGCGGGGGAAATCTTCGGTGGTGGGGTCAATGCGTTCTCCCGTTATGAAAACGGCAAGAC
CACCCCGCCAGTGGCGTTGGTGAAACTGCTCAAGCTGCTGGATCGCCATCCAGAACTCTTTGAGGAAGTGCGTACTGCCT
GA

Structures

Toxin

• AlphaFold DB: Q88F93

Antitoxin

• AlphaFold DB: I7AUJ7