Detailed information of TA system TA119861

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 1022602..1023197
• Replicon: chromosome
• Accession: NZ_CP036492
• Organism: Pseudomonas aeruginosa strain Paer4

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: EZZ78_RS04660
• Protein ID: WP_003113526.1
• Coordinates: 1022602..1022880 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: EZZ78_RS04665
• Protein ID: WP_003113527.1
• Coordinates: 1022892..1023197 (+)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EZZ78_RS04640 (EZZ78_04735)	1018028..1019266	+	1239	WP_003113524.1	dipeptidase	-
EZZ78_RS04645 (EZZ78_04740)	1019328..1019975	+	648	WP_003095021.1	carbonate dehydratase	-
EZZ78_RS04650 (EZZ78_04745)	1020045..1022273	-	2229	WP_003113525.1	TonB-dependent receptor	-
EZZ78_RS04655 (EZZ78_04750)	1022421..1022549	+	129	Protein_912	integrase	-
EZZ78_RS04660 (EZZ78_04755)	1022602..1022880	+	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EZZ78_RS04665 (EZZ78_04760)	1022892..1023197	+	306	WP_003113527.1	HigA family addiction module antitoxin	Antitoxin
EZZ78_RS04670 (EZZ78_04765)	1023561..1024634	-	1074	WP_003113528.1	serine/threonine-protein kinase	-
EZZ78_RS04675 (EZZ78_04770)	1024631..1025680	-	1050	WP_003113529.1	serine/threonine-protein kinase	-
EZZ78_RS04680 (EZZ78_04775)	1025683..1026780	-	1098	WP_169917322.1	protein phosphatase 2C domain-containing protein	-
EZZ78_RS04685 (EZZ78_04780)	1026915..1027184	-	270	WP_071536122.1	hypothetical protein	-
EZZ78_RS04690 (EZZ78_04785)	1027310..1027582	+	273	WP_003115921.1	hypothetical protein	-
EZZ78_RS04695 (EZZ78_04795)	1027794..1028084	+	291	WP_033992920.1	DUF5447 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (22-69)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T119861 WP_003113526.1 NZ_CP036492:1022602-1022880 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T119861 NZ_CP036492:1022602-1022880 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTACGGAGCTTCGAGACCTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11247.81 Da
• Isoelectric Point: 5.0747

>AT119861 WP_003113527.1 NZ_CP036492:1022892-1023197 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMEFDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT119861 NZ_CP036492:1022892-1023197 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTTGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCAAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Structures

Toxin

• AlphaFold DB: V6ALY3