Detailed information of TA system TA119799

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 3435022..3435619
• Replicon: chromosome
• Accession: NZ_CP036438
• Organism: Klebsiella pneumoniae strain ABFQB

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A9J6S5A1
• Locus tag: WN11_RS16955
• Protein ID: WP_004893639.1
• Coordinates: 3435022..3435339 (+)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: R4YH91
• Locus tag: WN11_RS16960
• Protein ID: WP_004142561.1
• Coordinates: 3435332..3435619 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
WN11_RS16935	3430726..3432153	-	1428	WP_004176980.1	MFS transporter	-
WN11_RS16940	3432262..3433128	+	867	WP_004151823.1	helix-turn-helix transcriptional regulator	-
WN11_RS16950	3433794..3434837	+	1044	WP_004893645.1	DUF2157 domain-containing protein	-
WN11_RS16955	3435022..3435339	+	318	WP_004893639.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
WN11_RS16960	3435332..3435619	+	288	WP_004142561.1	helix-turn-helix transcriptional regulator	Antitoxin
WN11_RS16965	3435884..3436537	-	654	WP_004178896.1	oxygen-insensitive NAD(P)H nitroreductase	-
WN11_RS16970	3436657..3437025	-	369	WP_004142557.1	MmcQ/YjbR family DNA-binding protein	-
WN11_RS16975	3437025..3437606	-	582	WP_004176968.1	TetR/AcrR family transcriptional regulator	-
WN11_RS16980	3437786..3438901	+	1116	WP_012737592.1	MBL fold metallo-hydrolase	-
WN11_RS16985	3438932..3439273	+	342	WP_002893035.1	RamA family antibiotic efflux transcriptional regulator	-
WN11_RS16990	3439291..3439539	-	249	WP_002893037.1	DUF1158 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (38-88)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12100.35 Da
• Isoelectric Point: 11.2767

>T119799 WP_004893639.1 NZ_CP036438:3435022-3435339 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPSIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Nucleotide

• Length: 318 bp

>T119799 NZ_CP036438:3435022-3435339 [Klebsiella pneumoniae]
ATGTTCAGATTGGTTGTGCATGTGGACGTTAAAAAGGAATTGCAGGCGCTTCCGTCCATTGTCCAGGCGAAGATGATCCG
GCAATTAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGACAGTAAACCGCTGGGGCAGGGGCTATTTGAAA
TTCGTGCGTTAGGTTCGGTACAGGGGCGAGGGATCTACGTTTTTCAGCAAGGCAAGACGCTCTTTCTGCTACGGGTTTTT
GTCAAGAAAACGCAAAAAACCCCGTCATCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAAATGCGCAATGAGTAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10771.46 Da
• Isoelectric Point: 10.2212

>AT119799 WP_004142561.1 NZ_CP036438:3435332-3435619 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Nucleotide

• Length: 288 bp

>AT119799 NZ_CP036438:3435332-3435619 [Klebsiella pneumoniae]
ATGAGTAAAAAGATCATTGACTGGGATACGTTAAAAACAGAACTGCTTGGCCAGCCTGAGGTTCAGGCTGCATTCGATGC
GCAAGAACGTCAACAGCGATTACGTACTATGCTGGCGCAATGGCGCGAGCAGGCGGGGCTAACCCGAGCCCAGGTGGCGC
AGAGGATGGGGGTGAGTGCGCCGACGGTCTCCAGAATGGAAGCCAATATCACCCGCGCCAGTCTGGATACCTTAACGCGA
TACGCGTTAGCCTGCGGTATAAAGCACCCGCAAGTTTTACTTTACTAA

Structures

Antitoxin

• AlphaFold DB: A0A0S3DIQ1