Detailed information of TA system TA119686

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 171674..171927
• Replicon: plasmid pKPC2_020037
• Accession: NZ_CP036372
• Organism: Klebsiella pneumoniae strain WCHKP020037

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: CEA76_RS28905
• Protein ID: WP_001312851.1
• Coordinates: 171778..171927 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	171674..171733 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CEA76_RS28860	167334..167399	-	66	Protein_212	helix-turn-helix domain-containing protein	-
CEA76_RS28865	167452..168156	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
CEA76_RS28870	168220..168381	+	162	Protein_214	DNA helicase	-
CEA76_RS28875	168401..169147	+	747	WP_000205770.1	conjugal transfer pilus acetylase TraX	-
CEA76_RS28880	169202..169762	+	561	WP_000139328.1	fertility inhibition protein FinO	-
CEA76_RS28885	169894..170094	+	201	WP_015059022.1	hypothetical protein	-
CEA76_RS28890	170480..171079	+	600	WP_032083981.1	hypothetical protein	-
CEA76_RS28895	171243..171473	+	231	WP_001736714.1	hypothetical protein	-
-	171674..171733	-	60	NuclAT_1	-	Antitoxin
-	171674..171733	-	60	NuclAT_1	-	Antitoxin
-	171674..171733	-	60	NuclAT_1	-	Antitoxin
-	171674..171733	-	60	NuclAT_1	-	Antitoxin
CEA76_RS28905	171778..171927	+	150	WP_001312851.1	type I toxin-antitoxin system Hok family toxin	Toxin
CEA76_RS28910	172211..172459	+	249	WP_000083837.1	replication regulatory protein RepA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCTX-M-65 / fosA3 / blaTEM-1B / rmtB / blaKPC-2 / catA2	-	1..172770	172770

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T119686 WP_001312851.1 NZ_CP036372:171778-171927 [Klebsiella pneumoniae]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T119686 NZ_CP036372:171778-171927 [Klebsiella pneumoniae]
ATGACGAAATATGCCCTTATCGGGTTGCTCGCCGTGTGTGCCACGGTATTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 60 bp

>AT119686 NZ_CP036372:c171733-171674 [Klebsiella pneumoniae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin