Detailed information of TA system TA119658

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 81553..81806
• Replicon: plasmid pCTXM65_115068
• Accession: NZ_CP036366
• Organism: Klebsiella pneumoniae strain WCHKP115068

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: DVJ72_RS28000
• Protein ID: WP_001312851.1
• Coordinates: 81657..81806 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	81553..81612 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DVJ72_RS27955	76929..77276	+	348	Protein_94	recombinase family protein	-
DVJ72_RS27960	77331..78035	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
DVJ72_RS27965	78099..78260	+	162	Protein_96	DNA helicase	-
DVJ72_RS27970	78280..79026	+	747	WP_000205770.1	conjugal transfer pilus acetylase TraX	-
DVJ72_RS27975	79081..79641	+	561	WP_000139328.1	fertility inhibition protein FinO	-
DVJ72_RS27980	79773..79973	+	201	WP_015059022.1	hypothetical protein	-
DVJ72_RS27985	80359..80958	+	600	WP_032083981.1	hypothetical protein	-
DVJ72_RS27990	81122..81352	+	231	WP_001736714.1	hypothetical protein	-
-	81553..81612	-	60	NuclAT_0	-	Antitoxin
-	81553..81612	-	60	NuclAT_0	-	Antitoxin
-	81553..81612	-	60	NuclAT_0	-	Antitoxin
-	81553..81612	-	60	NuclAT_0	-	Antitoxin
DVJ72_RS28000	81657..81806	+	150	WP_001312851.1	type I toxin-antitoxin system Hok family toxin	Toxin
DVJ72_RS28005	82090..82338	+	249	WP_000083837.1	replication regulatory protein RepA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaCTX-M-65 / catA2 / blaKPC-12	-	1..82649	82649

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T119658 WP_001312851.1 NZ_CP036366:81657-81806 [Klebsiella pneumoniae]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T119658 NZ_CP036366:81657-81806 [Klebsiella pneumoniae]
ATGACGAAATATGCCCTTATCGGGTTGCTCGCCGTGTGTGCCACGGTATTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 60 bp

>AT119658 NZ_CP036366:c81612-81553 [Klebsiella pneumoniae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin