Detailed information of TA system TA119635

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 19553..19806
• Replicon: plasmid pKPC2_095080
• Accession: NZ_CP036362
• Organism: Klebsiella pneumoniae strain WCHKP2080

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: B7D45_RS27505
• Protein ID: WP_001312851.1
• Coordinates: 19657..19806 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	19553..19612 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B7D45_RS27465	15331..16035	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
B7D45_RS27470	16099..16260	+	162	Protein_10	DNA helicase	-
B7D45_RS27475	16280..17026	+	747	WP_000205770.1	conjugal transfer pilus acetylase TraX	-
B7D45_RS27480	17081..17641	+	561	WP_000139328.1	fertility inhibition protein FinO	-
B7D45_RS27485	17773..17973	+	201	WP_015059022.1	hypothetical protein	-
B7D45_RS27490	18359..18958	+	600	WP_032083981.1	hypothetical protein	-
B7D45_RS27495	19122..19352	+	231	WP_001736714.1	hypothetical protein	-
-	19553..19612	-	60	NuclAT_0	-	Antitoxin
-	19553..19612	-	60	NuclAT_0	-	Antitoxin
-	19553..19612	-	60	NuclAT_0	-	Antitoxin
-	19553..19612	-	60	NuclAT_0	-	Antitoxin
B7D45_RS27505	19657..19806	+	150	WP_001312851.1	type I toxin-antitoxin system Hok family toxin	Toxin
B7D45_RS27510	20090..20338	+	249	WP_000083837.1	replication regulatory protein RepA	-
B7D45_RS27520	20583..20657	+	75	WP_001375168.1	RepA leader peptide Tap	-
B7D45_RS27525	20650..21507	+	858	WP_032495102.1	incFII family plasmid replication initiator RepA	-
B7D45_RS27535	22446..23099	+	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-
B7D45_RS27540	23192..23449	+	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
B7D45_RS27545	23382..23783	+	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-
B7D45_RS27550	24032..24447	+	416	Protein_23	IS1 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaCTX-M-65 / blaKPC-2	-	1..120195	120195
	-	flank	IS/Tn	-	-	15331..16035	704

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T119635 WP_001312851.1 NZ_CP036362:19657-19806 [Klebsiella pneumoniae]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T119635 NZ_CP036362:19657-19806 [Klebsiella pneumoniae]
ATGACGAAATATGCCCTTATCGGGTTGCTCGCCGTGTGTGCCACGGTATTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 60 bp

>AT119635 NZ_CP036362:c19612-19553 [Klebsiella pneumoniae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin