Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 2001567..2002164 Replicon chromosome
Accession NZ_CP036327
Organism Klebsiella pneumoniae strain BA28434

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag C3E96_RS10095 Protein ID WP_004142563.1
Coordinates 2001847..2002164 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag C3E96_RS10090 Protein ID WP_004142561.1
Coordinates 2001567..2001854 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C3E96_RS10060 1997647..1997895 + 249 WP_002893037.1 DUF1158 domain-containing protein -
C3E96_RS10065 1997913..1998254 - 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
C3E96_RS10070 1998285..1999400 - 1116 WP_012737592.1 MBL fold metallo-hydrolase -
C3E96_RS10075 1999580..2000164 + 585 WP_071579668.1 TetR/AcrR family transcriptional regulator -
C3E96_RS10080 2000161..2000529 + 369 WP_040171587.1 MmcQ/YjbR family DNA-binding protein -
C3E96_RS10085 2000649..2001302 + 654 WP_131082172.1 oxygen-insensitive NAD(P)H nitroreductase -
C3E96_RS10090 2001567..2001854 - 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
C3E96_RS10095 2001847..2002164 - 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
C3E96_RS10100 2002349..2003392 - 1044 WP_040153598.1 DUF2157 domain-containing protein -
C3E96_RS10110 2004062..2004928 - 867 WP_004151823.1 helix-turn-helix transcriptional regulator -
C3E96_RS10115 2005037..2006464 + 1428 WP_009308097.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T119576 WP_004142563.1 NZ_CP036327:c2002164-2001847 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T119576 NZ_CP036327:c2002164-2001847 [Klebsiella pneumoniae]
ATGTTCAGATTGGTTGTGCATGTGGACGTTAAAAAGGAATTGCAGGCGCTTCCGCCCATTGTCCAGGCGAAGATGATCCG
GCAATTAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGACAGTAAACCGCTGGGGCAGGGGCTATTTGAAA
TTCGTGCGTTAGGTTCGGTACAGGGGCGAGGGATCTACGTTTTTCAGCAAGGCAAGACGCTCTTTCTGCTACGGGTTTTT
GTCAAGAAAACGCAAAAAACCCCGTCATCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAGATGCGCAATGAGTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT119576 WP_004142561.1 NZ_CP036327:c2001854-2001567 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT119576 NZ_CP036327:c2001854-2001567 [Klebsiella pneumoniae]
ATGAGTAAAAAGATCATTGACTGGGATACGTTAAAAACAGAACTGCTTGGCCAGCCTGAGGTTCAGGCTGCATTCGATGC
GCAAGAACGTCAACAGCGATTACGTACTATGCTGGCGCAATGGCGCGAGCAGGCGGGGCTAACCCGAGCCCAGGTGGCGC
AGAGGATGGGGGTGAGTGCGCCGACGGTCTCCAGAATGGAAGCCAATATCACCCGCGCCAGTCTGGATACCTTAACGCGA
TACGCGTTAGCCTGCGGTATAAAGCACCCGCAAGTTTTACTTTACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References