Detailed information of TA system TA119534

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 52121..52848
• Replicon: plasmid p1_020098
• Accession: NZ_CP036307
• Organism: Klebsiella pneumoniae strain WCHKP020098

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A5Q9LNL2
• Locus tag: C6M05_RS28805
• Protein ID: WP_004118600.1
• Coordinates: 52121..52432 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: V0AHC4
• Locus tag: C6M05_RS28810
• Protein ID: WP_000990392.1
• Coordinates: 52429..52848 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6M05_RS28785	48635..49354	+	720	WP_001102107.1	(Fe-S)-binding protein	-
C6M05_RS28790	49365..50792	+	1428	WP_000023895.1	iron-sulfur cluster-binding protein	-
C6M05_RS28795	50785..51480	+	696	WP_004118595.1	lactate utilization protein C	-
C6M05_RS28800	51530..51910	-	381	Protein_48	gluconate permease	-
C6M05_RS28805	52121..52432	+	312	WP_004118600.1	type II toxin-antitoxin system HigB family toxin	Toxin
C6M05_RS28810	52429..52848	+	420	WP_000990392.1	helix-turn-helix domain-containing protein	Antitoxin
C6M05_RS28815	52885..54087	-	1203	WP_003031541.1	hypothetical protein	-
C6M05_RS28820	54080..54409	-	330	WP_004118613.1	hypothetical protein	-
C6M05_RS28825	54406..55065	-	660	WP_000078540.1	hypothetical protein	-
C6M05_RS31575	55489..56082	-	594	WP_044348674.1	hypothetical protein	-
C6M05_RS28840	56398..56751	+	354	WP_001114073.1	As(III)-sensing metalloregulatory transcriptional repressor ArsR	-
C6M05_RS28845	56799..57161	+	363	WP_020324593.1	arsenite efflux transporter metallochaperone ArsD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aac(3)-IId / blaLAP-2 / qnrS1 / sul1 / qacE / dfrA1 / tet(A)	-	1..229483	229483

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (20-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (83-136)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12386.14 Da
• Isoelectric Point: 10.0935

>T119534 WP_004118600.1 NZ_CP036307:52121-52432 [Klebsiella pneumoniae]
MHIVSRAPFDTATRQFPNQAAALDDVYRTLKRENYTSPDEMKKRFASLDRMKYREKWWVIDVGGGNLRVMFFADFERGKI
FIKHITTHAEYDKLTDFYRRTKE

Nucleotide

• Length: 312 bp

>T119534 NZ_CP036307:52121-52432 [Klebsiella pneumoniae]
ATGCACATTGTATCGAGAGCACCTTTCGATACAGCCACCAGGCAATTCCCCAATCAGGCAGCGGCACTAGACGACGTATA
CCGGACTTTGAAGAGAGAGAACTACACTTCACCGGACGAAATGAAAAAACGATTTGCCTCGCTGGACAGAATGAAATATC
GGGAGAAGTGGTGGGTGATCGATGTTGGCGGTGGGAATTTAAGGGTGATGTTTTTTGCTGATTTTGAACGTGGGAAAATC
TTTATAAAGCACATAACCACACACGCCGAATACGACAAGCTGACAGATTTTTACCGGAGAACTAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15534.71 Da
• Isoelectric Point: 4.4702

>AT119534 WP_000990392.1 NZ_CP036307:52429-52848 [Klebsiella pneumoniae]
MMYTDAIQAANSLVSIVPLLGGNASRKDYEDALTLVEYLVEHEPDHPLVDMLVAKIAQYEDEAEEFAEFNDRIAALPSGV
ALLRVLMDQHKLTQSDFEEEIGKKSLVSRILNGTRSLTLDHMKALARRFNIPPSSFMDA

Nucleotide

• Length: 420 bp

>AT119534 NZ_CP036307:52429-52848 [Klebsiella pneumoniae]
ATGATGTACACCGATGCTATCCAGGCGGCAAACAGCCTGGTTAGCATTGTCCCCCTCCTGGGGGGCAATGCCTCTCGCAA
GGACTATGAAGACGCGCTGACGCTGGTTGAATACCTGGTTGAACACGAGCCAGATCATCCGCTGGTGGACATGCTGGTTG
CGAAAATTGCCCAGTATGAAGATGAGGCGGAAGAGTTCGCAGAGTTCAACGACCGTATTGCAGCGTTACCAAGTGGCGTG
GCCCTGCTGCGTGTCCTGATGGACCAGCACAAACTGACTCAGAGTGATTTTGAGGAAGAGATTGGTAAGAAATCGCTGGT
GAGCCGCATCCTTAACGGGACTCGCTCACTAACACTAGATCACATGAAAGCACTTGCGCGGCGCTTCAACATTCCGCCCA
GCTCATTCATGGACGCGTAA

Structures

Toxin

• AlphaFold DB: A0A5Q9LNL2

Antitoxin

• AlphaFold DB: V0AHC4