Detailed information of TA system TA119526

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 52972..53398
• Replicon: plasmid pKPC2_020098
• Accession: NZ_CP036306
• Organism: Klebsiella pneumoniae strain WCHKP020098

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: C6M05_RS31235
• Protein ID: WP_001312861.1
• Coordinates: 52972..53130 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	53174..53398 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6M05_RS27720	48027..48254	-	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-
C6M05_RS27725	48348..49034	-	687	WP_015059009.1	PAS domain-containing protein	-
C6M05_RS27730	49225..49608	-	384	WP_000124981.1	relaxosome protein TraM	-
C6M05_RS27735	49885..50532	+	648	WP_015059008.1	transglycosylase SLT domain-containing protein	-
C6M05_RS27740	50829..51650	-	822	WP_001234445.1	DUF945 domain-containing protein	-
C6M05_RS27745	51761..52057	-	297	WP_001272251.1	hypothetical protein	-
C6M05_RS31560	52357..52734	+	378	Protein_70	hypothetical protein	-
C6M05_RS31235	52972..53130	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	53174..53398	-	225	NuclAT_0	-	Antitoxin
-	53174..53398	-	225	NuclAT_0	-	Antitoxin
-	53174..53398	-	225	NuclAT_0	-	Antitoxin
-	53174..53398	-	225	NuclAT_0	-	Antitoxin
C6M05_RS27770	53410..54129	-	720	WP_001276217.1	plasmid SOS inhibition protein A	-
C6M05_RS27775	54126..54560	-	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
C6M05_RS27780	54629..56652	-	2024	Protein_74	ParB/RepB/Spo0J family partition protein	-
C6M05_RS27785	56713..56946	-	234	WP_000006003.1	DUF905 family protein	-
C6M05_RS27790	57004..57531	-	528	WP_000290834.1	single-stranded DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaKPC-2 / blaSHV-12 / rmtB / blaTEM-1B / fosA3 / blaCTX-M-65	-	1..149519	149519

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T119526 WP_001312861.1 NZ_CP036306:c53130-52972 [Klebsiella pneumoniae]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T119526 NZ_CP036306:c53130-52972 [Klebsiella pneumoniae]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT119526 NZ_CP036306:c53398-53174 [Klebsiella pneumoniae]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin