Detailed information of TA system TA119500

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 153628..153881
• Replicon: plasmid pKPC2_015093
• Accession: NZ_CP036301
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain WCHKP015093

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: CLQ99_RS28635
• Protein ID: WP_001312851.1
• Coordinates: 153732..153881 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	153628..153687 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CLQ99_RS28590	149288..149353	-	66	Protein_188	helix-turn-helix domain-containing protein	-
CLQ99_RS28595	149406..150110	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
CLQ99_RS28600	150174..150335	+	162	Protein_190	DNA helicase	-
CLQ99_RS28605	150355..151101	+	747	WP_000205770.1	conjugal transfer pilus acetylase TraX	-
CLQ99_RS28610	151156..151716	+	561	WP_000139328.1	fertility inhibition protein FinO	-
CLQ99_RS28615	151848..152048	+	201	WP_015059022.1	hypothetical protein	-
CLQ99_RS28620	152434..153033	+	600	WP_032083981.1	hypothetical protein	-
CLQ99_RS28625	153197..153427	+	231	WP_001736714.1	hypothetical protein	-
-	153628..153687	-	60	NuclAT_1	-	Antitoxin
-	153628..153687	-	60	NuclAT_1	-	Antitoxin
-	153628..153687	-	60	NuclAT_1	-	Antitoxin
-	153628..153687	-	60	NuclAT_1	-	Antitoxin
CLQ99_RS28635	153732..153881	+	150	WP_001312851.1	type I toxin-antitoxin system Hok family toxin	Toxin
CLQ99_RS28640	154165..154413	+	249	WP_000083837.1	replication regulatory protein RepA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaKPC-2 / blaCTX-M-65 / blaTEM-1B / rmtB	-	1..154724	154724

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T119500 WP_001312851.1 NZ_CP036301:153732-153881 [Klebsiella pneumoniae subsp. pneumoniae]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T119500 NZ_CP036301:153732-153881 [Klebsiella pneumoniae subsp. pneumoniae]
ATGACGAAATATGCCCTTATCGGGTTGCTCGCCGTGTGTGCCACGGTATTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 60 bp

>AT119500 NZ_CP036301:c153687-153628 [Klebsiella pneumoniae subsp. pneumoniae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin