Detailed information of TA system TA119385

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 89544..89775
• Replicon: plasmid pS65EC
• Accession: NZ_CP036244
• Organism: Escherichia coli strain S65EC

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: A0A762TWR7
• Locus tag: S65EC_RS00595
• Protein ID: WP_023144756.1
• Coordinates: 89641..89775 (+)
• Length: 45 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	89544..89595 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
S65EC_RS00570	87074..87820	+	747	WP_000205718.1	conjugal transfer pilus acetylase TraX	-
S65EC_RS00575	87875..88435	+	561	WP_000139341.1	fertility inhibition protein FinO	-
S65EC_RS00580	88566..88778	+	213	WP_013023861.1	hypothetical protein	-
S65EC_RS26815	89291..89577	+	287	Protein_107	DUF2726 domain-containing protein	-
-	89544..89595	-	52	NuclAT_1	-	Antitoxin
-	89544..89595	-	52	NuclAT_1	-	Antitoxin
-	89544..89595	-	52	NuclAT_1	-	Antitoxin
-	89544..89595	-	52	NuclAT_1	-	Antitoxin
S65EC_RS00595	89641..89775	+	135	WP_023144756.1	type I toxin-antitoxin system Hok family toxin	Toxin
S65EC_RS00600	90072..90326	+	255	WP_000083850.1	replication regulatory protein RepA	-
S65EC_RS26975	90432..90566	+	135	Protein_110	protein CopA/IncA	-
S65EC_RS00605	90563..90637	+	75	WP_031943482.1	RepA leader peptide Tap	-
S65EC_RS00610	90630..91487	+	858	WP_032265352.1	incFII family plasmid replication initiator RepA	-
S65EC_RS26820	92191..92331	+	141	WP_165395303.1	hypothetical protein	-
S65EC_RS00625	92387..92641	+	255	WP_000108831.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	-
S65EC_RS00630	92631..92916	+	286	Protein_115	type II toxin-antitoxin system RelE/ParE family toxin	-
S65EC_RS00635	93282..93947	-	666	WP_086732345.1	EAL domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..146792	146792

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-44)

Sequences

Toxin

Protein

• Length: 45 a.a.
• Molecular weight: 4896.90 Da
• Isoelectric Point: 7.7209

>T119385 WP_023144756.1 NZ_CP036244:89641-89775 [Escherichia coli]
MTKYTLIGLLAVCATVLCFSLIFREQLCELNIHRGNTVVQVTLA

Nucleotide

• Length: 135 bp

>T119385 NZ_CP036244:89641-89775 [Escherichia coli]
ATGACGAAATATACCCTTATCGGGTTGCTTGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACAGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTGA

Antitoxin

Nucleotide

• Length: 52 bp

>AT119385 NZ_CP036244:c89595-89544 [Escherichia coli]
TCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A762TWR7

Antitoxin