Detailed information of TA system TA119381

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 48463..48733
• Replicon: plasmid pS65EC
• Accession: NZ_CP036244
• Organism: Escherichia coli strain S65EC

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: S65EC_RS00340
• Protein ID: WP_001312861.1
• Coordinates: 48575..48733 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	48463..48526 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
S65EC_RS26965	43751..44086	+	336	WP_014106969.1	hypothetical protein	-
S65EC_RS26970	43999..44205	+	207	WP_000275853.1	hypothetical protein	-
S65EC_RS00315	44231..44770	+	540	WP_000290841.1	single-stranded DNA-binding protein	-
S65EC_RS00320	44833..45066	+	234	WP_000005990.1	DUF905 family protein	-
S65EC_RS00325	45132..47090	+	1959	WP_086732397.1	ParB/RepB/Spo0J family partition protein	-
S65EC_RS00330	47145..47579	+	435	WP_000845940.1	conjugation system SOS inhibitor PsiB	-
S65EC_RS00335	47576..48295	+	720	WP_001276232.1	plasmid SOS inhibition protein A	-
S65EC_RS26810	48307..48495	-	189	WP_001299721.1	hypothetical protein	-
-	48307..48531	+	225	NuclAT_0	-	-
-	48307..48531	+	225	NuclAT_0	-	-
-	48307..48531	+	225	NuclAT_0	-	-
-	48307..48531	+	225	NuclAT_0	-	-
-	48463..48526	-	64	-	-	Antitoxin
S65EC_RS00340	48575..48733	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
S65EC_RS00360	49655..49942	+	288	WP_000107535.1	hypothetical protein	-
S65EC_RS00365	50062..50883	+	822	WP_001234469.1	DUF945 domain-containing protein	-
S65EC_RS00370	51180..51782	-	603	WP_000243712.1	transglycosylase SLT domain-containing protein	-
S65EC_RS00375	52113..52496	+	384	WP_001151566.1	relaxosome protein TraM	-
S65EC_RS00380	52630..53307	+	678	WP_001348626.1	PAS domain-containing protein	-
S65EC_RS00385	53395..53622	+	228	WP_001254388.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..146792	146792

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T119381 WP_001312861.1 NZ_CP036244:48575-48733 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T119381 NZ_CP036244:48575-48733 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 64 bp

>AT119381 NZ_CP036244:c48526-48463 [Escherichia coli]
GACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCTT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin