Detailed information of TA system TA119379

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 4525462..4526171
• Replicon: chromosome
• Accession: NZ_CP036220
• Organism: Mycobacterium avium subsp. hominissuis strain mc2 2500

Toxin (Protein)

• Gene name: HigB2
• Uniprot ID: X7V9P5
• Locus tag: EX350_RS20975
• Protein ID: WP_003873387.1
• Coordinates: 4525462..4525866 (+)
• Length: 135 a.a.

Antitoxin (Protein)

• Gene name: HigA2
• Uniprot ID: X7V856
• Locus tag: EX350_RS20980
• Protein ID: WP_019732445.1
• Coordinates: 4525863..4526171 (+)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EX350_RS20950	4520793..4521371	-	579	WP_011726026.1	sigma-70 family RNA polymerase sigma factor	-
EX350_RS20955	4521462..4522274	-	813	WP_170945464.1	hypothetical protein	-
EX350_RS20960	4522673..4522981	+	309	WP_003874400.1	WhiB family transcriptional regulator	-
EX350_RS20965	4523024..4523788	+	765	WP_023900746.1	superoxide dismutase family protein	-
EX350_RS20970	4523945..4525144	-	1200	WP_075362332.1	FAD-dependent oxidoreductase	-
EX350_RS20975	4525462..4525866	+	405	WP_003873387.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EX350_RS20980	4525863..4526171	+	309	WP_019732445.1	helix-turn-helix transcriptional regulator	Antitoxin
EX350_RS20985	4526658..4527017	-	360	Protein_4148	adenylate/guanylate cyclase domain-containing protein	-
EX350_RS20990	4527082..4528314	-	1233	WP_011725525.1	IS256-like element IS1245 family transposase	-
EX350_RS20995	4528435..4529607	-	1173	Protein_4150	adenylate/guanylate cyclase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	4527082..4528314	1232

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-109)

Antitoxin

• HigA2-like, helix-turn-helix domain (32-91)

Sequences

Toxin

Protein

• Length: 135 a.a.
• Molecular weight: 15503.82 Da
• Isoelectric Point: 10.3815

>T119379 WP_003873387.1 NZ_CP036220:4525462-4525866 [Mycobacterium avium subsp. hominissuis]
VRKWEVDLTLIEAWMDALDDEEYDNLIAALEQLEEHGPITRRPFVDTLEGSRHPNMKELRPRPTKAGAHIRVLFAFDTRS
RAIMLIAGDKAGNWSKWYAKHIPIADELFDAHQKRLHKAAAKATNRKPRKGKKR

Nucleotide

• Length: 405 bp

>T119379 NZ_CP036220:4525462-4525866 [Mycobacterium avium subsp. hominissuis]
GTGCGGAAATGGGAGGTTGACCTGACTCTCATCGAAGCCTGGATGGACGCCCTCGATGACGAGGAGTACGACAACTTGAT
AGCAGCGCTCGAGCAGTTGGAGGAACACGGTCCAATTACCCGGCGGCCGTTTGTGGACACCCTTGAAGGGTCACGGCACC
CGAACATGAAGGAGCTCCGTCCGCGCCCCACGAAAGCTGGAGCCCACATTCGCGTGCTATTCGCCTTCGATACCCGGTCG
CGGGCGATCATGTTGATCGCTGGAGACAAGGCGGGGAATTGGTCCAAGTGGTATGCCAAGCACATTCCCATTGCCGACGA
GCTCTTCGATGCTCACCAAAAGCGTTTGCACAAGGCAGCAGCCAAAGCCACAAATCGGAAACCAAGGAAGGGTAAGAAAC
GATGA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11454.95 Da
• Isoelectric Point: 10.0802

>AT119379 WP_019732445.1 NZ_CP036220:4525863-4526171 [Mycobacterium avium subsp. hominissuis]
MTNLDDMRRRRPGNRTRIDAIKAEMDREVAHYRLRELREAAGYTQTTLAAAIGVGQNRVSQMEHGDLGTSRVDTLRKYVE
ATGGELEVSVKRPDGSRVLLSL

Nucleotide

• Length: 309 bp

>AT119379 NZ_CP036220:4525863-4526171 [Mycobacterium avium subsp. hominissuis]
ATGACCAATCTTGATGACATGCGCCGTCGCCGTCCCGGCAACCGGACCCGTATCGACGCGATCAAGGCGGAGATGGACCG
CGAGGTTGCCCACTACCGTCTCCGTGAACTGCGCGAGGCTGCCGGCTACACGCAGACGACTCTCGCTGCGGCTATTGGCG
TCGGGCAGAACCGCGTCTCGCAGATGGAACATGGCGATCTCGGCACCAGCCGGGTCGACACTCTCCGCAAGTACGTGGAA
GCGACTGGGGGTGAGCTGGAAGTGTCCGTCAAGCGTCCGGACGGGTCACGGGTTCTCCTCAGTCTGTAA

Structures

Toxin

• AlphaFold DB: X7V9P5

Antitoxin

• AlphaFold DB: X7V856