Detailed information of TA system TA119366

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 13752..14016
• Replicon: plasmid punnamed1
• Accession: NZ_CP036203
• Organism: Escherichia coli strain L725

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: -
• Locus tag: D9K49_RS23715
• Protein ID: WP_024179505.1
• Coordinates: 13864..14016 (+)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	13752..13812 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D9K49_RS23700	8991..11282	-	2292	WP_130723486.1	conjugal transfer protein TrbC	-
D9K49_RS23705	11275..12345	-	1071	WP_124046954.1	IncI1-type conjugal transfer protein TrbB	-
D9K49_RS23710	12364..13572	-	1209	WP_130723487.1	IncI1-type conjugal transfer protein TrbA	-
-	13752..13812	-	61	NuclAT_0	-	Antitoxin
-	13752..13812	-	61	NuclAT_0	-	Antitoxin
-	13752..13812	-	61	NuclAT_0	-	Antitoxin
-	13752..13812	-	61	NuclAT_0	-	Antitoxin
D9K49_RS23715	13864..14016	+	153	WP_024179505.1	Hok/Gef family protein	Toxin
D9K49_RS23720	14088..14339	-	252	WP_024171163.1	hypothetical protein	-
-	14733..14784	-	52	NuclAT_1	-	-
-	14733..14784	-	52	NuclAT_1	-	-
-	14733..14784	-	52	NuclAT_1	-	-
-	14733..14784	-	52	NuclAT_1	-	-
D9K49_RS25295	15270..15446	-	177	WP_001054900.1	hypothetical protein	-
D9K49_RS23730	15629..15838	-	210	WP_001140544.1	hemolysin expression modulator Hha	-
D9K49_RS23735	15934..16596	-	663	WP_130723488.1	plasmid IncI1-type surface exclusion protein ExcA	-
D9K49_RS23740	16661..18829	-	2169	WP_130723489.1	DotA/TraY family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..93880	93880

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5743.07 Da
• Isoelectric Point: 8.7948

>T119366 WP_024179505.1 NZ_CP036203:13864-14016 [Escherichia coli]
MPQRTFLMMLIVVCVTILCFVWIVRDSLCGLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T119366 NZ_CP036203:13864-14016 [Escherichia coli]
ATGCCACAGCGAACGTTTTTAATGATGTTAATCGTCGTCTGTGTGACGATTCTGTGTTTTGTCTGGATTGTGAGGGATTC
GCTTTGCGGACTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin

Nucleotide

• Length: 61 bp

>AT119366 NZ_CP036203:c13812-13752 [Escherichia coli]
GAGGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Antitoxin