119355

Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 3381955..3382457 Replicon chromosome
Accession NZ_CP036202
Organism Escherichia coli strain L725

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag D9K49_RS16305 Protein ID WP_000767829.1
Coordinates 3382203..3382457 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag D9K49_RS16300 Protein ID WP_001259255.1
Coordinates 3381955..3382206 (+) Length 84 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D9K49_RS16275 3377133..3378200 - 1068 WP_000080102.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
D9K49_RS16280 3378200..3379270 - 1071 WP_000108941.1 histidinol-phosphate transaminase -
D9K49_RS16285 3379267..3380571 - 1305 WP_001542955.1 histidinol dehydrogenase -
D9K49_RS16290 3380577..3381476 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
D9K49_RS16295 3381622..3381672 - 51 WP_001364200.1 his operon leader peptide -
D9K49_RS16300 3381955..3382206 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
D9K49_RS16305 3382203..3382457 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
D9K49_RS16310 3382540..3383364 + 825 WP_000754758.1 SDR family oxidoreductase -
D9K49_RS16315 3383410..3384339 + 930 WP_000803351.1 LysR family transcriptional regulator -
D9K49_RS16320 3384554..3384616 + 63 WP_010723108.1 membrane protein YoeI -
D9K49_RS16325 3384606..3385964 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
D9K49_RS16330 3386143..3387201 + 1059 WP_000492346.1 transport protein YeeE -
D9K49_RS16335 3387215..3387442 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(2-84)

Antitoxin

(1-73)


Sequences


Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T119355 WP_000767829.1 NZ_CP036202:3382203-3382457 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T119355 NZ_CP036202:3382203-3382457 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT119355 WP_001259255.1 NZ_CP036202:3381955-3382206 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT119355 NZ_CP036202:3381955-3382206 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q


Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LNR0

References