Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 3381955..3382457 Replicon chromosome
Accession NZ_CP036202
Organism Escherichia coli strain L725

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag D9K49_RS16305 Protein ID WP_000767829.1
Coordinates 3382203..3382457 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag D9K49_RS16300 Protein ID WP_001259255.1
Coordinates 3381955..3382206 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D9K49_RS16275 3377133..3378200 - 1068 WP_000080102.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
D9K49_RS16280 3378200..3379270 - 1071 WP_000108941.1 histidinol-phosphate transaminase -
D9K49_RS16285 3379267..3380571 - 1305 WP_001542955.1 histidinol dehydrogenase -
D9K49_RS16290 3380577..3381476 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
D9K49_RS16295 3381622..3381672 - 51 WP_001364200.1 his operon leader peptide -
D9K49_RS16300 3381955..3382206 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
D9K49_RS16305 3382203..3382457 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
D9K49_RS16310 3382540..3383364 + 825 WP_000754758.1 SDR family oxidoreductase -
D9K49_RS16315 3383410..3384339 + 930 WP_000803351.1 LysR family transcriptional regulator -
D9K49_RS16320 3384554..3384616 + 63 WP_010723108.1 membrane protein YoeI -
D9K49_RS16325 3384606..3385964 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
D9K49_RS16330 3386143..3387201 + 1059 WP_000492346.1 transport protein YeeE -
D9K49_RS16335 3387215..3387442 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T119355 WP_000767829.1 NZ_CP036202:3382203-3382457 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T119355 NZ_CP036202:3382203-3382457 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT119355 WP_001259255.1 NZ_CP036202:3381955-3382206 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT119355 NZ_CP036202:3381955-3382206 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References