Detailed information of TA system TA119350

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 2101406..2102205
• Replicon: chromosome
• Accession: NZ_CP036202
• Organism: Escherichia coli strain L725

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: -
• Locus tag: D9K49_RS10135
• Protein ID: WP_000347253.1
• Coordinates: 2101406..2101870 (-)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: -
• Locus tag: D9K49_RS10140
• Protein ID: WP_024251361.1
• Coordinates: 2101870..2102205 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D9K49_RS10105	2096407..2096841	-	435	WP_000948824.1	PTS sugar transporter subunit IIA	-
D9K49_RS10110	2096859..2097737	-	879	WP_001295548.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
D9K49_RS10115	2097727..2098506	-	780	WP_000406214.1	PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC	-
D9K49_RS10120	2098517..2098990	-	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
D9K49_RS10125	2099013..2100293	-	1281	WP_000681910.1	tagatose-bisphosphate aldolase subunit KbaZ	-
D9K49_RS10130	2100542..2101351	+	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
D9K49_RS10135	2101406..2101870	-	465	WP_000347253.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
D9K49_RS10140	2101870..2102205	-	336	WP_024251361.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
D9K49_RS10145	2102354..2103925	-	1572	WP_001273766.1	galactarate dehydratase	-
D9K49_RS10150	2104300..2105646	+	1347	WP_130723372.1	galactarate/glucarate/glycerate transporter GarP	-
D9K49_RS10155	2105650..2106420	+	771	WP_001058226.1	2-dehydro-3-deoxyglucarate aldolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17764.14 Da
• Isoelectric Point: 9.4947

>T119350 WP_000347253.1 NZ_CP036202:c2101870-2101406 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEETH

Nucleotide

• Length: 465 bp

>T119350 NZ_CP036202:c2101870-2101406 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGATGCTTTAGTTGC
CGAAGTCGAGGCATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTGGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGCTATCGTCTCTTCTTTCGTTACAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGTAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAAACAGAAGAAACCCATTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12348.90 Da
• Isoelectric Point: 4.8616

>AT119350 WP_024251361.1 NZ_CP036202:c2102205-2101870 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
SQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT119350 NZ_CP036202:c2102205-2101870 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCAGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
TCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACGATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
TCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA