Detailed information of TA system TA119342

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 4324619..4325268
• Replicon: chromosome
• Accession: NZ_CP036199
• Organism: Leclercia adecarboxylata strain 16005813

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A855EN38
• Locus tag: EXN74_RS20925
• Protein ID: WP_032616921.1
• Coordinates: 4324619..4324981 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: EXN74_RS20930
• Protein ID: WP_059308573.1
• Coordinates: 4324969..4325268 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EXN74_RS20905	4319873..4320853	-	981	WP_032616924.1	LacI family transcriptional regulator	-
EXN74_RS20910	4320929..4322071	-	1143	WP_130589208.1	Gfo/Idh/MocA family oxidoreductase	-
EXN74_RS20915	4322064..4323089	-	1026	WP_059308572.1	sugar phosphate isomerase/epimerase	-
EXN74_RS20920	4323101..4324342	-	1242	WP_032616942.1	MFS transporter	-
EXN74_RS20925	4324619..4324981	+	363	WP_032616921.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EXN74_RS20930	4324969..4325268	+	300	WP_059308573.1	helix-turn-helix transcriptional regulator	Antitoxin
EXN74_RS20935	4325409..4326794	-	1386	WP_077226217.1	phenylalanine transporter	-
EXN74_RS20940	4326898..4328871	-	1974	WP_130589209.1	PhoX family phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-107)

Antitoxin

• HigA2-like, helix-turn-helix domain (12-87)

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 14014.97 Da
• Isoelectric Point: 6.4838

>T119342 WP_032616921.1 NZ_CP036199:4324619-4324981 [Leclercia adecarboxylata]
MWEVETTDTFDKWFNHQTQALKEDVLAIMHILSEYGPQLGRPWVDTVKNSHYANMKELRIQHAGDPVRAFFAFDPVRKAI
ILCAGNKTGKNEKRFYKDMITMADAEFSDYLASKEAEWLR

Nucleotide

• Length: 363 bp

>T119342 NZ_CP036199:4324619-4324981 [Leclercia adecarboxylata]
ATGTGGGAAGTCGAAACAACGGATACGTTTGACAAGTGGTTTAACCACCAGACACAAGCCTTGAAAGAAGATGTCCTGGC
TATAATGCATATCCTCTCAGAGTACGGTCCACAGCTGGGCCGTCCCTGGGTCGATACAGTTAAAAACTCACATTACGCGA
ATATGAAAGAGCTACGCATTCAGCATGCAGGGGATCCGGTTCGTGCTTTTTTTGCCTTCGATCCTGTTCGTAAAGCCATA
ATTTTATGTGCTGGAAATAAGACGGGAAAAAATGAAAAGCGATTCTATAAAGATATGATCACCATGGCTGACGCTGAATT
CAGCGATTATCTGGCGAGTAAGGAGGCCGAATGGCTACGTTAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10972.68 Da
• Isoelectric Point: 5.8526

>AT119342 WP_059308573.1 NZ_CP036199:4324969-4325268 [Leclercia adecarboxylata]
MATLKELMAQQSQESQERIAAKVEDLRIIVALNQLREELNISQTELAAAMGVKQPTVAKIEQPDNDPRLSTLKRYVTALG
GEVSIDVILPTGKRVAFHF

Nucleotide

• Length: 300 bp

>AT119342 NZ_CP036199:4324969-4325268 [Leclercia adecarboxylata]
ATGGCTACGTTAAAAGAACTCATGGCCCAGCAGAGCCAGGAAAGTCAGGAGCGAATTGCCGCTAAAGTTGAGGATCTGCG
CATTATTGTCGCGCTCAACCAGCTCCGGGAAGAACTCAACATATCGCAAACTGAACTTGCTGCCGCGATGGGGGTTAAAC
AGCCTACCGTTGCGAAAATTGAGCAACCGGATAATGATCCGCGTCTTTCTACGCTTAAACGCTATGTTACGGCATTAGGG
GGTGAAGTGAGTATTGATGTCATTCTGCCCACCGGGAAGCGGGTGGCGTTTCATTTTTGA

Structures

Toxin

• AlphaFold DB: A0A855EN38