Detailed information of TA system TA119339

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3000579..3001306
• Replicon: chromosome
• Accession: NZ_CP036199
• Organism: Leclercia adecarboxylata strain 16005813

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A482LZ08
• Locus tag: EXN74_RS14580
• Protein ID: WP_045898923.1
• Coordinates: 3000995..3001306 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: EXN74_RS14575
• Protein ID: WP_103181184.1
• Coordinates: 3000579..3000998 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EXN74_RS14535	2995658..2996557	+	900	WP_130588774.1	dihydrodipicolinate synthase family protein	-
EXN74_RS14540	2996784..2997203	+	420	WP_032610985.1	translesion error-prone DNA polymerase V autoproteolytic subunit	-
EXN74_RS14545	2997397..2998074	+	678	WP_051915977.1	DUF1571 domain-containing protein	-
EXN74_RS14550	2998089..2998565	+	477	WP_103792261.1	SRPBCC family protein	-
EXN74_RS14555	2998818..2998919	+	102	Protein_2766	hypothetical protein	-
EXN74_RS14565	2999247..3000394	+	1148	WP_130588775.1	IS3 family transposase	-
EXN74_RS14570	3000408..3000601	+	194	Protein_2768	hypothetical protein	-
EXN74_RS14575	3000579..3000998	-	420	WP_103181184.1	helix-turn-helix domain-containing protein	Antitoxin
EXN74_RS14580	3000995..3001306	-	312	WP_045898923.1	type II toxin-antitoxin system HigB family toxin	Toxin
EXN74_RS14585	3002355..3002753	-	399	WP_103181183.1	H-NS histone family protein	-
EXN74_RS14590	3003613..3004026	-	414	WP_130588776.1	hypothetical protein	-
EXN74_RS14595	3004679..3005092	+	414	WP_103181181.1	hypothetical protein	-
EXN74_RS14600	3005137..3005274	+	138	Protein_2774	hypothetical protein	-
EXN74_RS14605	3005280..3005954	+	675	Protein_2775	efflux RND transporter permease subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	2981249..3042005	60756

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12347.07 Da
• Isoelectric Point: 9.7248

>T119339 WP_045898923.1 NZ_CP036199:c3001306-3000995 [Leclercia adecarboxylata]
MHVISKEPFEEAAKQYPNDSLAVRALYRLVRETDFSSPAEMRTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T119339 NZ_CP036199:c3001306-3000995 [Leclercia adecarboxylata]
ATGCACGTAATTTCAAAAGAGCCTTTTGAGGAAGCGGCAAAGCAATATCCCAACGATTCGTTAGCCGTTCGGGCGCTGTA
CCGCTTAGTCCGCGAGACGGACTTTTCTTCTCCAGCTGAGATGCGAACGCTGATACCGAGCCTGGACAATTTTAAGTATC
GTAATAAGTGGTGGGTGTTGGATGTAGGGGGCAACAACTTGAGGGTTATCGCCTATATCAATTTCGTGAATAAACGCTTC
TATGTGAAGCACATCGCCACCCACGCTGATTACGACAAGTTGACCCGCTATTACAGGGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15475.62 Da
• Isoelectric Point: 4.3573

>AT119339 WP_103181184.1 NZ_CP036199:c3000998-3000579 [Leclercia adecarboxylata]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAIELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEMPV
GVALLRTLIDQYRLSYSDLKEEIGSKSLVSQILSGQRSLTIMHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT119339 NZ_CP036199:c3000998-3000579 [Leclercia adecarboxylata]
ATGATTACCGACACTGCAAAAGCCATTGAAGCAACCAAACAACTCGTAGCTGCTGTTCCCTTCCTGGGGGGCAGTTCGTC
CGAGAGCGATTACCGCGAAGCGATAGAGTTGGTGGACTATCTGATCGAAAACGACGACGAGAACCCGCTTATTGACTTCC
TGGCGAGTAAAATTGCTGACTATGAGGATAACAGCCCTCGTTTCGCTGAATTCAACAAGGCTATCGCTGAAATGCCAGTC
GGCGTTGCCCTTCTGCGCACCCTGATTGACCAGTACAGGCTGTCTTATTCTGACCTTAAGGAGGAGATCGGCTCTAAATC
GCTGGTTAGCCAGATTCTCTCTGGTCAGCGGTCACTGACAATCATGCACATTAAGGCGCTGTCTGCTCGTTTTGGTGTCA
AACCAGAGTGGTTCCTTTGA

Structures

Toxin

• AlphaFold DB: A0A482LZ08